MolNFT v1 · #179254 · protein + RNA

Cryoem structure of a CRISPR-CAS complex

7JZY · CRISPR-associated protein CSY1: “Cryoem structure of a CRISPR-CAS complex”. Electron microscopy, 20 pockets (best druggability 0.85). MolNFT v1 #179254 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 7JZY
Type: protein + RNA
Length: 3,084 residues
Chains: 12
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: CRISPR-associated protein CSY1; Type I-F CRISPR-associated protein CSY2; Type I-F…
UniProt: Q02ML9, B3G161, A0A643HZS6, A0A444M080
Pockets: 20 · best druggability 0.85

Structure

electron microscopy · 8 distinct chains · 29% helix, 22% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	CRISPR-associated protein CSY1	protein	427	45% helix, 6% strand	Q02ML9
B	Type I-F CRISPR-associated protein CSY2	protein	306	23% helix, 29% strand	B3G161
C	Type I-F CRISPR-associated endoribonuclease CAS6/CSY4	protein	187	26% helix, 25% strand	A0A643HZS6
M	RNA (61-mer)	rna	60	—	—
D	CRISPR type I-F/YPEST-associated protein CSY3	protein	298	25% helix, 25% strand	A0A444M080
E, F, G, I	CRISPR type I-F/YPEST-associated protein CSY3	protein	335	25% helix, 24% strand	A0A444M080
H	CRISPR type I-F/YPEST-associated protein CSY3	protein	330	25% helix, 25% strand	A0A444M080
J, K	ACRF9	protein	68	15% helix, 38% strand	—

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	985	0.85	0.86	112	empty
2	1187	0.83	0.85	131	empty
3	468	0.83	0.90	60	empty
4	1012	0.82	0.84	106	empty
5	689	0.82	0.86	80	empty

Interfaces

34 interfaces between chains.

A–B · 4641 Å² buried · 53 hydrogen bonds · protein-protein
H–I · 2074 Å² buried · 29 hydrogen bonds · protein-protein
F–G · 2066 Å² buried · 23 hydrogen bonds · protein-protein
B–I · 2047 Å² buried · 26 hydrogen bonds · protein-protein
G–H · 2039 Å² buried · 24 hydrogen bonds · protein-protein

Compared structures

7JZY · CryoEM structure of a CRISPR-Cas complex · the same entry in another collection · chain K against K, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q02ML9
Every structure of UniProt B3G161
Every structure of UniProt A0A643HZS6
Every structure of UniProt A0A444M080
Elsewhere: RCSB PDB 7JZY · PDBe 7JZY · UniProt Q02ML9 · UniProt B3G161 · UniProt A0A643HZS6 · UniProt A0A444M080

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