Research workbench
Cryoem structure of a CRISPR-CAS complex
Analyse Cryoem structure of a CRISPR-CAS complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7JZY
- Type
- protein + RNA
- Length
- 3,084 residues
- Chains
- 12
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR-associated protein CSY1; Type I-F CRISPR-associated protein CSY2; Type I-F…
- UniProt
- Q02ML9, B3G161, A0A643HZS6, A0A444M080
- Pockets
- 20 · best druggability 0.85
Structure
electron microscopy · 8 distinct chains · 29% helix, 22% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | CRISPR-associated protein CSY1 | protein | 427 | 45% helix, 6% strand | Q02ML9 |
| B | Type I-F CRISPR-associated protein CSY2 | protein | 306 | 23% helix, 29% strand | B3G161 |
| C | Type I-F CRISPR-associated endoribonuclease CAS6/CSY4 | protein | 187 | 26% helix, 25% strand | A0A643HZS6 |
| M | RNA (61-mer) | rna | 60 | — | — |
| D | CRISPR type I-F/YPEST-associated protein CSY3 | protein | 298 | 25% helix, 25% strand | A0A444M080 |
| E, F, G, I | CRISPR type I-F/YPEST-associated protein CSY3 | protein | 335 | 25% helix, 24% strand | A0A444M080 |
| H | CRISPR type I-F/YPEST-associated protein CSY3 | protein | 330 | 25% helix, 25% strand | A0A444M080 |
| J, K | ACRF9 | protein | 68 | 15% helix, 38% strand | — |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 985 | 0.85 | 0.86 | 112 | empty |
| 2 | 1187 | 0.83 | 0.85 | 131 | empty |
| 3 | 468 | 0.83 | 0.90 | 60 | empty |
| 4 | 1012 | 0.82 | 0.84 | 106 | empty |
| 5 | 689 | 0.82 | 0.86 | 80 | empty |
Interfaces
34 interfaces between chains.
- A–B · 4641 Ų buried · 53 hydrogen bonds · protein-protein
- H–I · 2074 Ų buried · 29 hydrogen bonds · protein-protein
- F–G · 2066 Ų buried · 23 hydrogen bonds · protein-protein
- B–I · 2047 Ų buried · 26 hydrogen bonds · protein-protein
- G–H · 2039 Ų buried · 24 hydrogen bonds · protein-protein
Compared structures
- 7JZY · CryoEM structure of a CRISPR-Cas complex · the same entry in another collection · chain K against K, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q02ML9
- Every structure of UniProt B3G161
- Every structure of UniProt A0A643HZS6
- Every structure of UniProt A0A444M080
- Elsewhere: RCSB PDB 7JZY · PDBe 7JZY · UniProt Q02ML9 · UniProt B3G161 · UniProt A0A643HZS6 · UniProt A0A444M080
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