MolNFT v1 · #179252 · protein + RNA
Cryo-EM structure of CRISPR-CAS surveillance complex with ACRIF4
7JZW · CRISPR type I-F/YPEST-associated protein CSY1: “Cryo-EM structure of CRISPR-CAS surveillance complex with ACRIF4”. Electron microscopy, 20 pockets (best druggability 0.81). MolNFT v1 #179252 on GenesisL1: 3D structure, binding sites…
- PDB
- 7JZW
- Type
- protein + RNA
- Length
- 3,046 residues
- Chains
- 11
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR type I-F/YPEST-associated protein CSY1; CRISPR-associated endonuclease CAS6/CSY4…
- EC
- 3.1.-.-
- UniProt
- A0A643HYU6, Q02MM2, B3G161, A0A444M080, A0A076FR21
- Pockets
- 20 · best druggability 0.81
Structure
electron microscopy · 8 distinct chains · 29% helix, 22% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | CRISPR type I-F/YPEST-associated protein CSY1 | protein | 427 | 44% helix, 6% strand | A0A643HYU6 |
| C | CRISPR-associated endonuclease CAS6/CSY4 (EC 3.1.-.-) | protein | 187 | 25% helix, 25% strand | Q02MM2 |
| B | CRISPR type I-F/YPEST-associated protein CSY2 | protein | 306 | 22% helix, 31% strand | B3G161 |
| E, F, G, I | CRISPR type I-F/YPEST-associated protein CSY3 | protein | 335 | 24% helix, 25% strand | A0A444M080 |
| D | CRISPR type I-F/YPEST-associated protein CSY3 | protein | 298 | 24% helix, 29% strand | A0A444M080 |
| H | CRISPR type I-F/YPEST-associated protein CSY3 | protein | 330 | 25% helix, 26% strand | A0A444M080 |
| J | Type I-F anti-CRISPR protein | protein | 98 | 46% helix, 21% strand | A0A076FR21 |
| M | CRISPR repeat sequence | rna | 60 | — | — |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 420 | 0.81 | 0.90 | 57 | empty |
| 2 | 1014 | 0.79 | 0.81 | 109 | empty |
| 3 | 852 | 0.79 | 0.82 | 101 | empty |
| 4 | 518 | 0.77 | 0.84 | 52 | empty |
| 5 | 784 | 0.76 | 0.81 | 82 | empty |
Interfaces
30 interfaces between chains.
- A–B · 4746 Ų buried · 65 hydrogen bonds · protein-protein
- G–H · 2120 Ų buried · 31 hydrogen bonds · protein-protein
- B–I · 2095 Ų buried · 37 hydrogen bonds · protein-protein
- H–I · 2088 Ų buried · 35 hydrogen bonds · protein-protein
- E–D · 2082 Ų buried · 32 hydrogen bonds · protein-protein
Compared structures
- 7JZW · Cryo-EM structure of CRISPR-Cas surveillance complex with AcrIF4 · the same entry in another collection · chain M against M, TM-score 1.00
- Q02MM2 · AlphaFold monomer V2.0 prediction for CRISPR-associated endonuclease CAS6/CSY4 (Q02MM2) · a predicted model of the same protein · chain C against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt A0A643HYU6
- Every structure of UniProt Q02MM2
- Every structure of UniProt B3G161
- Every structure of UniProt A0A444M080
- Every structure of UniProt A0A076FR21
- Elsewhere: RCSB PDB 7JZW · PDBe 7JZW · UniProt A0A643HYU6 · UniProt Q02MM2 · UniProt B3G161 · UniProt A0A444M080 · UniProt A0A076FR21
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