MolNFT v1 · #174369 · protein

Lifeact-F-actin complex

7BTE · Actin, alpha skeletal muscle: “Lifeact-F-actin complex”. Electron microscopy, 2 ligands (ADP, MG), 20 pockets (best druggability 0.82). MolNFT v1 #174369 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 7BTE
Type: protein
Length: 1,894 residues
Chains: 8
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Actin, alpha skeletal muscle; Lifeact
UniProt: P68139
Ligands: ADP, MG
Pockets: 20 · best druggability 0.82

Structure

electron microscopy · 3 distinct chains · 36% helix, 12% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, C, E, G, I	Actin, alpha skeletal muscle	protein	370	37% helix, 13% strand	P68139
L	Lifeact	protein	14	0% helix, 0% strand	—
M, N	Lifeact	protein	15	33% helix, 0% strand	—

Ligands and binding sites

ADP · Adenosine-5'-diphosphate · ligand · 5 copies · chains A, C, E, G, I
MG · Magnesium ion · metal · 5 copies · chains A, C, E, G, I

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 14 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	930	0.82	0.83	94	empty
2	309	0.80	0.91	53	MG, ADP
3	355	0.79	0.89	51	MG, ADP
4	431	0.78	0.87	54	MG, ADP
5	296	0.75	0.87	43	MG, ADP

Interfaces

13 interfaces between chains.

E–I · 1267 Å² buried · 11 hydrogen bonds · protein-protein
C–G · 1260 Å² buried · 10 hydrogen bonds · protein-protein
A–E · 1227 Å² buried · 6 hydrogen bonds · protein-protein
E–G · 489 Å² buried · 7 hydrogen bonds · protein-protein
A–C · 488 Å² buried · 5 hydrogen bonds · protein-protein

Compared structures

7BTE · Lifeact-F-actin complex · the same entry in another collection · chain I against I, TM-score 1.00
P68139 · AlphaFold monomer V2.0 prediction for actin, alpha skeletal muscle (P68139) · a predicted model of the same protein · chain A against A, TM-score 0.96

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P68139
Elsewhere: RCSB PDB 7BTE · PDBe 7BTE · UniProt P68139

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