MolNFT v1 · #15062 · protein + DNA + RNA
Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase
1MSW · Template DNA: “Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase”. X-ray diffraction, 14 pockets (best druggability 0.78). MolNFT v1 #15062 on GenesisL1: 3D structure, binding sites…
- PDB
- 1MSW
- Type
- protein + DNA + RNA
- Length
- 910 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Template DNA; Non-tempate DNA; RNA message; DNA-directed RNA polymerase
- EC
- 2.7.7.6
- UniProt
- P00573
- Pockets
- 14 · best druggability 0.78
Structure
x-ray diffraction · 4 distinct chains · 52% helix, 7% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| T | Template DNA | dna | 20 | — | — |
| N | Non-tempate DNA | dna | 17 | — | — |
| R | RNA message | rna | 10 | — | — |
| D | DNA-directed RNA polymerase (EC 2.7.7.6) | protein | 863 | 52% helix, 7% strand | P00573 |
Pockets
14 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 664 | 0.78 | 0.84 | 67 | empty |
| 2 | 932 | 0.77 | 0.80 | 81 | empty |
| 3 | 286 | 0.76 | 0.89 | 36 | empty |
| 4 | 878 | 0.75 | 0.79 | 68 | empty |
| 5 | 225 | 0.62 | 0.82 | 29 | empty |
Interfaces
5 interfaces between chains.
- T–D · 1057 Ų buried · 17 hydrogen bonds · protein-nucleic
- N–D · 1032 Ų buried · 8 hydrogen bonds · protein-nucleic
- R–D · 945 Ų buried · 13 hydrogen bonds · protein-nucleic
- T–N · 503 Ų buried · 31 hydrogen bonds · nucleic-nucleic
- T–R · 349 Ų buried · 31 hydrogen bonds · nucleic-nucleic
Compared structures
- 1MSW · Structural basis for the transition from initiation to elongation transcription in T7 RNA… · the same entry in another collection · chain T against T, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P00573
- Elsewhere: RCSB PDB 1MSW · PDBe 1MSW · UniProt P00573
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