Research workbench
Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase
Analyse Structural basis for the transition from initiation to elongation transcription… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1MSW
- Type
- protein + DNA + RNA
- Length
- 910 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Template DNA; Non-tempate DNA; RNA message; DNA-directed RNA polymerase
- EC
- 2.7.7.6
- UniProt
- P00573
- Pockets
- 14 · best druggability 0.78
Structure
x-ray diffraction · 4 distinct chains · 52% helix, 7% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| T | Template DNA | dna | 20 | — | — |
| N | Non-tempate DNA | dna | 17 | — | — |
| R | RNA message | rna | 10 | — | — |
| D | DNA-directed RNA polymerase (EC 2.7.7.6) | protein | 863 | 52% helix, 7% strand | P00573 |
Pockets
14 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 664 | 0.78 | 0.84 | 67 | empty |
| 2 | 932 | 0.77 | 0.80 | 81 | empty |
| 3 | 286 | 0.76 | 0.89 | 36 | empty |
| 4 | 878 | 0.75 | 0.79 | 68 | empty |
| 5 | 225 | 0.62 | 0.82 | 29 | empty |
Interfaces
5 interfaces between chains.
- T–D · 1057 Ų buried · 17 hydrogen bonds · protein-nucleic
- N–D · 1032 Ų buried · 8 hydrogen bonds · protein-nucleic
- R–D · 945 Ų buried · 13 hydrogen bonds · protein-nucleic
- T–N · 503 Ų buried · 31 hydrogen bonds · nucleic-nucleic
- T–R · 349 Ų buried · 31 hydrogen bonds · nucleic-nucleic
Compared structures
- 1MSW · Structural basis for the transition from initiation to elongation transcription in T7 RNA… · the same entry in another collection · chain T against T, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P00573
- Elsewhere: RCSB PDB 1MSW · PDBe 1MSW · UniProt P00573
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.