MolNFT v1 · #142487 · protein

Structure of 14-3-3 gamma in complex with tfeb 14-3-3 binding motif

6A5S · 14-3-3 protein gamma: “Structure of 14-3-3 gamma in complex with tfeb 14-3-3 binding motif”. X-ray diffraction, 2 ligands (NA, MG), 10 pockets (best druggability 0.78). MolNFT v1 #142487 on GenesisL1: 3D structure, binding sites and…

PDB: 6A5S
Type: protein
Length: 964 residues
Chains: 8
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: 14-3-3 protein gamma; Tfeb PS211-peptide
UniProt: P61981
Ligands: NA, MG
Pockets: 10 · best druggability 0.78

Structure

x-ray diffraction · 5 distinct chains · 82% helix, 0% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	14-3-3 protein gamma	protein	230	84% helix, 0% strand	P61981
E, C, F, H	Tfeb PS211-peptide	protein	11	0% helix, 0% strand	—
B	14-3-3 protein gamma	protein	229	84% helix, 0% strand	P61981
D	14-3-3 protein gamma	protein	233	82% helix, 0% strand	P61981
G	14-3-3 protein gamma	protein	228	84% helix, 0% strand	P61981

Ligands and binding sites

NA · Sodium ion · metal · chains A
MG · Magnesium ion · metal · chains B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

10 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1196	0.78	0.80	102	empty
2	65	0.69	0.95	24	empty
3	65	0.68	0.95	22	empty
4	66	0.68	0.94	23	empty
5	332	0.56	0.76	26	empty

Interfaces

8 interfaces between chains.

A–B · 1189 Å² buried · 15 hydrogen bonds · protein-protein
D–G · 1161 Å² buried · 13 hydrogen bonds · protein-protein
A–E · 822 Å² buried · 20 hydrogen bonds · protein-protein
G–H · 819 Å² buried · 18 hydrogen bonds · protein-protein
D–F · 815 Å² buried · 19 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 4 modified (phosphorylation).

Compared structures

6A5S · Structure of 14-3-3 gamma in complex with TFEB 14-3-3 binding motif · the same entry in another collection · chain H against H, TM-score 1.00
P61981 · AlphaFold monomer V2.0 prediction for 14-3-3 protein gamma (P61981) · a predicted model of the same protein · chain B against A, TM-score 0.99

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P61981
Elsewhere: RCSB PDB 6A5S · PDBe 6A5S · UniProt P61981

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