MolNFT v1 · #139635 · protein + RNA
Anti-CRISPR proteins ACRF1/2 bound to csy surveillance complex with a 32NT spacer crrna backbone region
5XLO · CRISPR-associated protein CSY3: “Anti-CRISPR proteins ACRF1/2 bound to csy surveillance complex with a 32NT spacer crrna backbone region”. Electron microscopy, 20 pockets (best druggability 0.84). MolNFT v1 #139635 on GenesisL1: 3D…
- PDB
- 5XLO
- Type
- protein + RNA
- Length
- 2,111 residues
- Chains
- 9
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR-associated protein CSY3; Crrna with 32NT spacer sequence; Uncharacterized protein…
- UniProt
- Q02MM1, L7P7M1
- Pockets
- 20 · best druggability 0.84
Structure
electron microscopy · 4 distinct chains · 25% helix, 13% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | CRISPR-associated protein CSY3 | protein | 287 | 25% helix, 11% strand | Q02MM1 |
| B, C, D, E, F | CRISPR-associated protein CSY3 | protein | 326 | 25% helix, 13% strand | Q02MM1 |
| K | Crrna with 32NT spacer sequence | rna | 38 | — | — |
| N, M | Uncharacterized protein ACRF1 | protein | 78 | 27% helix, 17% strand | L7P7M1 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1190 | 0.84 | 0.85 | 115 | empty |
| 2 | 959 | 0.84 | 0.85 | 93 | empty |
| 3 | 992 | 0.82 | 0.84 | 93 | empty |
| 4 | 849 | 0.80 | 0.83 | 93 | empty |
| 5 | 978 | 0.79 | 0.81 | 88 | empty |
Interfaces
17 interfaces between chains.
- D–E · 1719 Ų buried · 13 hydrogen bonds · protein-protein
- C–D · 1718 Ų buried · 10 hydrogen bonds · protein-protein
- E–F · 1717 Ų buried · 9 hydrogen bonds · protein-protein
- B–C · 1689 Ų buried · 11 hydrogen bonds · protein-protein
- A–B · 1677 Ų buried · 11 hydrogen bonds · protein-protein
Compared structures
- 5XLO · Anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 32nt spacer crRNA… · the same entry in another collection · chain M against M, TM-score 1.00
- Q02MM1 · AlphaFold monomer V2.0 prediction for CRISPR-associated protein CSY3 (Q02MM1) · a predicted model of the same protein · chain A against A, TM-score 0.96
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q02MM1
- Every structure of UniProt L7P7M1
- Elsewhere: RCSB PDB 5XLO · PDBe 5XLO · UniProt Q02MM1 · UniProt L7P7M1
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