MolNFT v1 · #12576 · protein
An engineered streptavidin with improved affinity for the strep-tag II peptide : apo-SAM2
1KL4: “An engineered streptavidin with improved affinity for the strep-tag II peptide : apo-SAM2”. X-ray diffraction, 3 pockets (best druggability 0.73). MolNFT v1 #12576 on GenesisL1: 3D structure, binding sites and pockets in GL1X.
- PDB
- 1KL4
- Type
- protein
- Length
- 478 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Streptavidin
- UniProt
- P22629
- Pockets
- 3 · best druggability 0.73
Structure
x-ray diffraction · 3 distinct chains · 8% helix, 56% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Streptavidin | protein | 120 | 8% helix, 57% strand | P22629 |
| B | Streptavidin | protein | 120 | 8% helix, 56% strand | P22629 |
| C, D | Streptavidin | protein | 119 | 8% helix, 56% strand | P22629 |
Pockets
3 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 864 | 0.73 | 0.78 | 90 | empty |
| 2 | 431 | 0.65 | 0.78 | 49 | empty |
| 3 | 395 | 0.64 | 0.78 | 46 | empty |
Interfaces
6 interfaces between chains.
- A–B · 1605 Ų buried · 23 hydrogen bonds · protein-protein
- C–D · 1591 Ų buried · 20 hydrogen bonds · protein-protein
- B–C · 408 Ų buried · 2 hydrogen bonds · protein-protein
- A–D · 404 Ų buried · 2 hydrogen bonds · protein-protein
- B–D · 204 Ų buried · 2 hydrogen bonds · protein-protein
Compared structures
- 1KL4 · AN ENGINEERED STREPTAVIDIN WITH IMPROVED AFFINITY FOR THE STREP-TAG II PEPTIDE : apo-SAM2 · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P22629
- Elsewhere: RCSB PDB 1KL4 · PDBe 1KL4 · UniProt P22629
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