X-ray crystal structure at 2.31A resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydroquinoline compound and an internal aldimine linked plp cofactor.

Structure

x-ray diffraction · 2 distinct chains · 26% helix, 31% strand (DSSP).

Ligands and binding sites

• PLP · Pyridoxal-5'-phosphate · ligand · 2 copies · chains A, B
• 67X · (3R)-3-methyl-1,2,3,4-tetrahydroquinoline-8-sulfonamide · ligand · 2 copies · chains A, B
• CL · Chloride ion · ion · 4 copies · chains A, B
• EDO · 1,2-ethanediol · additive · 7 copies · chains A, B
• DMS · Dimethyl sulfoxide · additive · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

6 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 2363 Ų buried · 33 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 2 cofactor (pyridoxal phosphate Schiff base).

Compared structures

• 5I5T · X-ray crystal structure at 2.31A resolution of human mitochondrial branched chain… · the same entry in another collection · chain A against A, TM-score 1.00
• O15382 · AlphaFold monomer V2.0 prediction for branched-chain-amino-acid aminotransferase… · a predicted model of the same protein · chain B against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt O15382
• Elsewhere: RCSB PDB 5I5T · PDBe 5I5T · UniProt O15382