MolNFT v1 · #121256 · protein

Insulin with proline analog hyp at position B28 in the R6 state

5HPU · Insulin, chain a: “Insulin with proline analog hyp at position B28 in the R6 state”. X-ray diffraction, 3 ligands (IPH, ZN, CL). MolNFT v1 #121256 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 5HPU
Type: protein
Length: 97 residues
Chains: 4
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Insulin, chain a; Insulin, chain B
UniProt: P01308
Ligands: IPH, ZN, CL

Structure

x-ray diffraction · 3 distinct chains · 71% helix, 9% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, C	Insulin, chain a	protein	21	62% helix, 5% strand	P01308
B	Insulin, chain B	protein	27	74% helix, 11% strand	P01308
D	Insulin, chain B	protein	28	75% helix, 11% strand	P01308

Ligands and binding sites

IPH · Phenol · ligand · 2 copies · chains A, B, C, D
ZN · Zinc ion · metal · 2 copies · chains B, D
CL · Chloride ion · ion · 2 copies · chains B, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

5 interfaces between chains.

B–D · 728 Å² buried · 11 hydrogen bonds · protein-protein
A–B · 582 Å² buried · 1 hydrogen bond · protein-protein
C–D · 560 Å² buried · 5 hydrogen bonds · protein-protein
A–D · 51 Å² buried · 0 hydrogen bonds · protein-protein
B–C · 12 Å² buried · 0 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 6 disulfide, 2 modified (disulfide, hydroxylation).

Compared structures

5HPU · Insulin with proline analog HyP at position B28 in the R6 state · the same entry in another collection · chain A against A, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P01308
Elsewhere: RCSB PDB 5HPU · PDBe 5HPU · UniProt P01308

GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.