MolNFT v1 · #115098 · protein + RNA
Structural basis of template boundary definition in tetrahymena telomerase
5C9H · Telomerase reverse transcriptase: “Structural basis of template boundary definition in tetrahymena telomerase”. X-ray diffraction, 1 ligand (MG), 12 pockets (best druggability 0.51). MolNFT v1 #115098 on GenesisL1: 3D structure…
- PDB
- 5C9H
- Type
- protein + RNA
- Length
- 512 residues
- Chains
- 6
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Telomerase reverse transcriptase; RNA (5'-R(P*AP*GP*AP*AP*CP*UP*GP*UP*CP*A)-3'); RNA…
- EC
- 2.7.7.49
- UniProt
- O77448
- Ligands
- MG
- Pockets
- 12 · best druggability 0.51
Structure
x-ray diffraction · 6 distinct chains · 54% helix, 5% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Telomerase reverse transcriptase (EC 2.7.7.49) | protein | 245 | 53% helix, 5% strand | O77448 |
| B | Telomerase reverse transcriptase (EC 2.7.7.49) | protein | 225 | 55% helix, 5% strand | O77448 |
| D | RNA (5'-R(P*AP*GP*AP*AP*CP*UP*GP*UP*CP*A)-3') | rna | 9 | — | — |
| C | RNA (5'-R(P*UP*CP*AP*UP*UP*CP*AP*GP*UP*UP*CP*U)-3') | rna | 12 | — | — |
| E | RNA (5'-R(P*AP*GP*AP*AP*CP*UP*GP*UP*CP*A)-3') | rna | 10 | — | — |
| G | RNA (5'-R(P*UP*CP*AP*UP*UP*CP*AP*GP*UP*UP*CP*U)-3') | rna | 11 | — | — |
Ligands and binding sites
- MG · Magnesium ion · metal · 2 copies · chains C, G
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
12 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 291 | 0.51 | 0.74 | 26 | MG |
| 2 | 99 | 0.45 | 0.79 | 22 | empty |
| 3 | 102 | 0.45 | 0.79 | 30 | empty |
| 4 | 107 | 0.43 | 0.77 | 18 | empty |
| 5 | 117 | 0.42 | 0.76 | 24 | empty |
Interfaces
7 interfaces between chains.
- A–C · 703 Ų buried · 22 hydrogen bonds · protein-nucleic
- B–G · 583 Ų buried · 7 hydrogen bonds · protein-nucleic
- A–B · 545 Ų buried · 6 hydrogen bonds · protein-protein
- B–E · 416 Ų buried · 13 hydrogen bonds · protein-nucleic
- A–D · 380 Ų buried · 8 hydrogen bonds · protein-nucleic
Compared structures
- 5C9H · Structural Basis of Template Boundary Definition in Tetrahymena Telomerase · the same entry in another collection · chain G against G, TM-score 1.00
- O77448 · AlphaFold monomer V2.0 prediction for telomerase reverse transcriptase (O77448) · a predicted model of the same protein · chain B against A, TM-score 0.95
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt O77448
- Elsewhere: RCSB PDB 5C9H · PDBe 5C9H · UniProt O77448
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