Human platelet phosphofructokinase in an R-state in complex with ADP and F6P, crystal form I

Structure

x-ray diffraction · 4 distinct chains · 48% helix, 19% strand (DSSP).

Ligands and binding sites

• FBP · 1,6-di-O-phosphono-beta-D-fructofuranose · ligand · 4 copies · chains A, B, C, D
• ADP · Adenosine-5'-diphosphate · ligand · 2 copies · chains B, D
• F6P · 6-O-phosphono-beta-D-fructofuranose · ligand · 2 copies · chains A, B, C, D
• PO4 · Phosphate ion · ion · 6 copies · chains A, B, C, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Interfaces

4 interfaces between chains.

• C–D · 2314 Ų buried · 42 hydrogen bonds · protein-protein
• A–B · 2247 Ų buried · 40 hydrogen bonds · protein-protein
• A–D · 792 Ų buried · 15 hydrogen bonds · protein-protein
• B–C · 750 Ų buried · 12 hydrogen bonds · protein-protein

Compared structures

• 4XZ2 · Human platelet phosphofructokinase in an R-state in complex with ADP and F6P, crystal… · the same entry in another collection · chain D against D, TM-score 1.00
• Q01813 · AlphaFold monomer V2.0 prediction for ATP-dependent 6-phosphofructokinase, platelet type… · a predicted model of the same protein · chain B against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt Q01813
• Elsewhere: RCSB PDB 4XZ2 · PDBe 4XZ2 · UniProt Q01813