Research workbench
Crystal structure of S. aureus pyruvate carboxylase A610T mutant
Analyse Crystal structure of S. aureus pyruvate carboxylase A610T mutant in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3HB9
- Type
- protein
- Length
- 4,267 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Pyruvate carboxylase
- UniProt
- Q99UY8
- Ligands
- BTI, ADP, MN
- Pockets
- 20 · best druggability 0.85
Structure
x-ray diffraction · 4 distinct chains · 38% helix, 19% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Pyruvate carboxylase | protein | 1133 | 37% helix, 19% strand | Q99UY8 |
| B | Pyruvate carboxylase | protein | 1074 | 37% helix, 19% strand | Q99UY8 |
| C | Pyruvate carboxylase | protein | 1067 | 39% helix, 18% strand | Q99UY8 |
| D | Pyruvate carboxylase | protein | 993 | 40% helix, 18% strand | Q99UY8 |
Ligands and binding sites
- BTI · 5-(hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-6-yl) pentanal · ligand · 4 copies · chains A, B, C, D
- ADP · Adenosine-5'-diphosphate · ligand · 2 copies · chains A, C
- MN · Manganese (II) ion · metal · 4 copies · chains A, B, C, D
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 865 | 0.85 | 0.88 | 92 | empty |
| 2 | 826 | 0.85 | 0.87 | 94 | empty |
| 3 | 1132 | 0.82 | 0.84 | 103 | empty |
| 4 | 1078 | 0.80 | 0.81 | 84 | ADP |
| 5 | 707 | 0.80 | 0.84 | 71 | empty |
Interfaces
6 interfaces between chains.
- B–D · 2999 Ų buried · 39 hydrogen bonds · protein-protein
- A–C · 2955 Ų buried · 40 hydrogen bonds · protein-protein
- A–D · 1616 Ų buried · 24 hydrogen bonds · protein-protein
- B–C · 1529 Ų buried · 22 hydrogen bonds · protein-protein
- A–B · 1002 Ų buried · 9 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 cofactor (biotinylation).
Compared structures
- 3HB9 · Crystal Structure of S. aureus Pyruvate Carboxylase A610T Mutant · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q99UY8
- Elsewhere: RCSB PDB 3HB9 · PDBe 3HB9 · UniProt Q99UY8
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