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Azurin mutant M121A

Analyse Azurin mutant M121A in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 2TSA
Type: protein
Length: 512 residues
Chains: 4
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Azurin
UniProt: P00282
Ligands: CU
Pockets: 4 · best druggability 0.74

Structure

x-ray diffraction · 1 distinct chain · 15% helix, 34% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, B, C, D	Azurin	protein	128	15% helix, 34% strand	P00282

Ligands and binding sites

CU · Copper (II) ion · metal · 4 copies · chains A, B, C, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

4 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	348	0.74	0.86	38	empty
2	259	0.71	0.86	27	empty
3	270	0.65	0.82	49	empty
4	205	0.62	0.83	35	empty

Interfaces

6 interfaces between chains.

A–C · 504 Å² buried · 0 hydrogen bonds · protein-protein
B–D · 484 Å² buried · 0 hydrogen bonds · protein-protein
A–D · 414 Å² buried · 5 hydrogen bonds · protein-protein
B–C · 407 Å² buried · 3 hydrogen bonds · protein-protein
C–D · 357 Å² buried · 1 hydrogen bond · protein-protein

Modifications

Observed in the structure: 4 disulfide (disulfide).

Compared structures

2TSA · Azurin mutant M121A · the same entry in another collection · chain B against B, TM-score 1.00
P00282 · AlphaFold monomer V2.0 prediction for azurin (P00282) · a predicted model of the same protein · chain C against A, TM-score 0.99

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P00282
Elsewhere: RCSB PDB 2TSA · PDBe 2TSA · UniProt P00282

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