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Crystal structure of E.COLI rseb

Analyse Crystal structure of E.COLI rseb in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 2P4B
Type: protein
Length: 804 residues
Chains: 3
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Sigma-E factor regulatory protein rseb
UniProt: P0AFX9
Pockets: 6 · best druggability 0.88

Structure

x-ray diffraction · 3 distinct chains · 13% helix, 48% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Sigma-E factor regulatory protein rseb	protein	284	13% helix, 50% strand	P0AFX9
B	Sigma-E factor regulatory protein rseb	protein	260	10% helix, 46% strand	P0AFX9
C	Sigma-E factor regulatory protein rseb	protein	260	15% helix, 48% strand	P0AFX9

Ligands and binding sites

BOG · octyl beta-D-glucopyranoside · additive · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

6 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	709	0.88	0.92	102	BOG
2	302	0.82	0.92	55	empty
3	52	0.59	0.92	16	empty
4	242	0.59	0.80	39	empty
5	70	0.41	0.80	15	empty

Interfaces

2 interfaces between chains.

A–B · 1909 Å² buried · 10 hydrogen bonds · protein-protein
A–C · 885 Å² buried · 25 hydrogen bonds · protein-protein

Compared structures

2P4B · Crystal structure of E.coli RseB · the same entry in another collection · chain B against B, TM-score 1.00
P0AFX9 · AlphaFold monomer V2.0 prediction for sigma-E factor regulatory protein rseb (P0AFX9) · a predicted model of the same protein · chain A against A, TM-score 0.98

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P0AFX9
Elsewhere: RCSB PDB 2P4B · PDBe 2P4B · UniProt P0AFX9

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