Crystal structure of the E. COLI ammonia channel amtb complexed with the signal transduction protein glnk

Structure

x-ray diffraction · 2 distinct chains · 63% helix, 9% strand (DSSP).

Ligands and binding sites

• ADP · Adenosine-5'-diphosphate · ligand · chains B
• BOG · octyl beta-D-glucopyranoside · additive · 8 copies · chains A
• TRS · 2-amino-2-hydroxymethyl-propane-1,3-diol · additive · 2 copies · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

3 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 532 Ų buried · 7 hydrogen bonds · protein-protein

Compared structures

• 2NS1 · Crystal structure of the e. coli ammonia channel AMTB complexed with the signal… · the same entry in another collection · chain A against A, TM-score 1.00
• P69681 · AlphaFold monomer V2.0 prediction for ammonia channel (P69681) · a predicted model of the same protein · chain A against A, TM-score 1.00
• P0AC55 · AlphaFold monomer V2.0 prediction for nitrogen regulatory protein P-II 2 (P0AC55) · a predicted model of the same protein · chain B against A, TM-score 0.95

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P69681
• Every structure of UniProt P0AC55
• Elsewhere: RCSB PDB 2NS1 · PDBe 2NS1 · UniProt P69681 · UniProt P0AC55