Research workbench
The structure of the complex of cytochrome P450CAM and its electron donor putidaredoxin determined by paramagnetic NMR spectroscopy
Analyse The structure of the complex of cytochrome P450CAM and its electron donor… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 2M56
- Type
- protein
- Length
- 510 residues
- Chains
- 2
- Method
- solution nmr
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Camphor 5-monooxygenase; Putidaredoxin
- EC
- 1.14.15.1
- UniProt
- P00183, P00259
- Ligands
- HEM, CAM, FES
- Pockets
- 4 · best druggability 0.86
Structure
solution nmr · 2 distinct chains · 44% helix, 13% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Camphor 5-monooxygenase (EC 1.14.15.1) | protein | 404 | 50% helix, 10% strand | P00183 |
| B | Putidaredoxin | protein | 106 | 19% helix, 22% strand | P00259 |
Ligands and binding sites
- HEM · Protoporphyrin IX containing Fe · ligand · chains A
- CAM · Camphor · ligand · chains A
- FES · Fe2/s2 (inorganic) cluster · ligand · chains B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
4 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 464 | 0.86 | 0.93 | 48 | HEM, CAM |
| 2 | 433 | 0.58 | 0.75 | 49 | empty |
| 3 | 99 | 0.42 | 0.78 | 22 | empty |
| 4 | 54 | 0.31 | 0.77 | 16 | empty |
Interfaces
1 interface between chains.
- A–B · 552 Ų buried · 11 hydrogen bonds · protein-protein
Compared structures
- 2M56 · The structure of the complex of cytochrome P450cam and its electron donor putidaredoxin… · the same entry in another collection · chain A against A, TM-score 1.00
- P00183 · AlphaFold monomer V2.0 prediction for camphor 5-monooxygenase (P00183) · a predicted model of the same protein · chain A against A, TM-score 1.00
- P00259 · AlphaFold monomer V2.0 prediction for putidaredoxin (P00259) · a predicted model of the same protein · chain B against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P00183
- Every structure of UniProt P00259
- Elsewhere: RCSB PDB 2M56 · PDBe 2M56 · UniProt P00183 · UniProt P00259
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