Research workbench
Structure of a guiderna-binding protein complex bound to a grna
Analyse Structure of a guiderna-binding protein complex bound to a grna in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 2GJE
- Type
- protein + RNA
- Length
- 310 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Guide RNA 40-mer; RNA tetramer; Mitochondrial RNA-binding protein 2; Mitochondrial…
- UniProt
- Q952G2, P90629
- Pockets
- 6 · best druggability 0.64
Structure
x-ray diffraction · 4 distinct chains · 25% helix, 39% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| R | Guide RNA 40-mer | rna | 13 | — | — |
| S | RNA tetramer | rna | 5 | — | — |
| A | Mitochondrial RNA-binding protein 2 | protein | 145 | 24% helix, 40% strand | Q952G2 |
| D | Mitochondrial RNA-binding protein 1 | protein | 147 | 25% helix, 39% strand | P90629 |
Pockets
6 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 333 | 0.64 | 0.80 | 24 | empty |
| 2 | 321 | 0.54 | 0.75 | 38 | empty |
| 3 | 110 | 0.39 | 0.76 | 15 | empty |
| 4 | 73 | 0.35 | 0.77 | 16 | empty |
| 5 | 91 | 0.31 | 0.72 | 18 | empty |
Interfaces
5 interfaces between chains.
- A–D · 1187 Ų buried · 9 hydrogen bonds · protein-protein
- R–D · 450 Ų buried · 7 hydrogen bonds · protein-nucleic
- R–A · 440 Ų buried · 8 hydrogen bonds · protein-nucleic
- R–S · 206 Ų buried · 11 hydrogen bonds · nucleic-nucleic
- S–A · 81 Ų buried · 0 hydrogen bonds · protein-nucleic
Modifications
Observed in the structure: 6 modified (engineered).
Compared structures
- 2GJE · Structure of a guideRNA-binding protein complex bound to a gRNA · the same entry in another collection · chain R against R, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q952G2
- Every structure of UniProt P90629
- Elsewhere: RCSB PDB 2GJE · PDBe 2GJE · UniProt Q952G2 · UniProt P90629
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.