Research workbench
Crystal structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from E. COLI
Analyse Crystal structure of the regulatory subunit of acetohydroxyacid synthase… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 2F1F
- Type
- protein
- Length
- 321 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Acetolactate synthase isozyme III small subunit
- EC
- 2.2.1.6
- UniProt
- P00894
- Ligands
- MG
- Pockets
- 4 · best druggability 0.59
Structure
x-ray diffraction · 2 distinct chains · 29% helix, 42% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Acetolactate synthase isozyme III small subunit (EC 2.2.1.6) | protein | 163 | 30% helix, 40% strand | P00894 |
| B | Acetolactate synthase isozyme III small subunit (EC 2.2.1.6) | protein | 158 | 28% helix, 43% strand | P00894 |
Ligands and binding sites
- MG · Magnesium ion · metal · 6 copies · chains A, B
- 1PE · Pentaethylene glycol · additive · chains A
- P33 · 3,6,9,12,15,18-hexaoxaicosane-1,20-diol · additive · chains B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
4 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 51 | 0.59 | 0.92 | 20 | empty |
| 2 | 79 | 0.44 | 0.80 | 14 | empty |
| 3 | 148 | 0.44 | 0.76 | 25 | empty |
| 4 | 93 | 0.38 | 0.76 | 21 | empty |
Interfaces
1 interface between chains.
- A–B · 2212 Ų buried · 25 hydrogen bonds · protein-protein
Compared structures
- 2F1F · Crystal structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from… · the same entry in another collection · chain A against A, TM-score 1.00
- P00894 · AlphaFold monomer V2.0 prediction for acetolactate synthase isozyme 3 small subunit… · a predicted model of the same protein · chain A against A, TM-score 0.97
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P00894
- Elsewhere: RCSB PDB 2F1F · PDBe 2F1F · UniProt P00894
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.