Research workbench
Crystal structure of a T7 RNA polymerase-T7 promoter complex
Analyse Crystal structure of a T7 RNA polymerase-T7 promoter complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1CEZ
- Type
- protein + DNA
- Length
- 894 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- DNA (5'- D(P*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3'); DNA (5'…
- EC
- 2.7.7.6
- UniProt
- P00573
- Pockets
- 12 · best druggability 0.82
Structure
x-ray diffraction · 3 distinct chains · 50% helix, 9% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| T | DNA (5'- D(P*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3') | dna | 17 | — | — |
| N | DNA (5'- D(P*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*TP*A)-3') | dna | 15 | — | — |
| A | Protein (bacteriophage T7 RNA polymerase) (EC 2.7.7.6) | protein | 862 | 50% helix, 9% strand | P00573 |
Pockets
12 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1170 | 0.82 | 0.83 | 110 | empty |
| 2 | 402 | 0.70 | 0.82 | 47 | empty |
| 3 | 270 | 0.56 | 0.77 | 41 | empty |
| 4 | 126 | 0.52 | 0.81 | 28 | empty |
| 5 | 165 | 0.50 | 0.78 | 27 | empty |
Interfaces
3 interfaces between chains.
- T–A · 1394 Ų buried · 37 hydrogen bonds · protein-nucleic
- N–A · 715 Ų buried · 12 hydrogen bonds · protein-nucleic
- T–N · 559 Ų buried · 38 hydrogen bonds · nucleic-nucleic
Compared structures
- 1CEZ · Crystal structure of a T7 RNA polymerase-T7 promoter complex · the same entry in another collection · chain T against T, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P00573
- Elsewhere: RCSB PDB 1CEZ · PDBe 1CEZ · UniProt P00573
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.