The crystal structure of biotin synthase, an S-adenosylmethionine- dependent radical enzyme

Structure

x-ray diffraction · 2 distinct chains · 41% helix, 15% strand (DSSP).

Ligands and binding sites

• SAM · S-adenosylmethionine · ligand · 2 copies · chains A, B
• SF4 · Iron/sulfur cluster · ligand · 2 copies · chains A, B
• FES · Fe2/s2 (inorganic) cluster · ligand · 2 copies · chains A, B
• DTB · 6-(5-methyl-2-oxo-imidazolidin-4-yl)-hexanoic acid · ligand · 2 copies · chains A, B
• TRS · 2-amino-2-hydroxymethyl-propane-1,3-diol · additive · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

4 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 1212 Ų buried · 10 hydrogen bonds · protein-protein

Compared structures

• 1R30 · The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme · the same entry in another collection · chain A against A, TM-score 1.00
• P12996 · AlphaFold monomer V2.0 prediction for biotin synthase (P12996) · a predicted model of the same protein · chain A against A, TM-score 0.98

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P12996
• Elsewhere: RCSB PDB 1R30 · PDBe 1R30 · UniProt P12996