Research workbench
Staphylococcus epidermidis RP62A CRISPR effector subcomplex
Analyse Staphylococcus epidermidis RP62A CRISPR effector subcomplex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7UZW
- Type
- protein + RNA
- Length
- 1,791 residues
- Chains
- 8
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR system cms endoribonuclease CSM3; CRISPR system cms protein CSM4; CRISPR system…
- UniProt
- Q5HK91, Q5HK92, Q5HK89
- Pockets
- 20 · best druggability 0.81
Structure
electron microscopy · 6 distinct chains · 36% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, C, D | CRISPR system cms endoribonuclease CSM3 | protein | 204 | 26% helix, 33% strand | Q5HK91 |
| B | CRISPR system cms endoribonuclease CSM3 | protein | 193 | 26% helix, 32% strand | Q5HK91 |
| E | CRISPR system cms endoribonuclease CSM3 | protein | 184 | 29% helix, 24% strand | Q5HK91 |
| H | CRISPR system cms protein CSM4 | protein | 293 | 24% helix, 23% strand | Q5HK92 |
| F | CRISPR system single-strand-specific deoxyribonuclease CAS10/CSM1 (subtype III-a) | protein | 478 | 53% helix, 10% strand | Q5HK89 |
| G | Crrna | rna | 31 | — | — |
Pockets
20 pockets found on a 1 Å grid, 17 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 847 | 0.81 | 0.83 | 65 | empty |
| 2 | 952 | 0.78 | 0.80 | 105 | empty |
| 3 | 62 | 0.73 | 0.97 | 19 | empty |
| 4 | 366 | 0.72 | 0.84 | 34 | empty |
| 5 | 334 | 0.71 | 0.84 | 37 | empty |
Interfaces
17 interfaces between chains.
- H–G · 1597 Ų buried · 27 hydrogen bonds · protein-nucleic
- D–E · 1459 Ų buried · 13 hydrogen bonds · protein-protein
- C–D · 1371 Ų buried · 9 hydrogen bonds · protein-protein
- A–B · 1344 Ų buried · 11 hydrogen bonds · protein-protein
- A–C · 1323 Ų buried · 7 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 1 disulfide (disulfide).
Compared structures
- 7UZW · Staphylococcus epidermidis RP62a CRISPR effector subcomplex · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q5HK91
- Every structure of UniProt Q5HK92
- Every structure of UniProt Q5HK89
- Elsewhere: RCSB PDB 7UZW · PDBe 7UZW · UniProt Q5HK91 · UniProt Q5HK92 · UniProt Q5HK89
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