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Basis for a switch in substrate specificity: crystal structure of selected variant of cre site-specific recombinase, alshg bound to the engineered recognition site LOXM7
Analyse Basis for a switch in substrate specificity: crystal structure of selected… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1PVP
- Type
- protein + DNA
- Length
- 707 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- 34-mer; Recombinase cre
- UniProt
- P06956
- Pockets
- 11 · best druggability 0.80
Structure
x-ray diffraction · 4 distinct chains · 56% helix, 6% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| C | 34-mer | dna | 32 | — | — |
| D | 34-mer | dna | 34 | — | — |
| A | Recombinase cre | protein | 323 | 56% helix, 4% strand | P06956 |
| B | Recombinase cre | protein | 318 | 57% helix, 9% strand | P06956 |
Pockets
11 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 678 | 0.80 | 0.85 | 69 | empty |
| 2 | 334 | 0.64 | 0.80 | 39 | empty |
| 3 | 246 | 0.59 | 0.80 | 28 | empty |
| 4 | 309 | 0.58 | 0.77 | 30 | empty |
| 5 | 72 | 0.47 | 0.83 | 22 | empty |
Interfaces
6 interfaces between chains.
- A–B · 1539 Ų buried · 25 hydrogen bonds · protein-protein
- C–B · 1500 Ų buried · 39 hydrogen bonds · protein-nucleic
- D–B · 1375 Ų buried · 27 hydrogen bonds · protein-nucleic
- D–A · 1290 Ų buried · 26 hydrogen bonds · protein-nucleic
- C–D · 712 Ų buried · 73 hydrogen bonds · nucleic-nucleic
Compared structures
- 1PVP · Basis for a switch in substrate specificity: crystal structure of selected variant of cre… · the same entry in another collection · chain C against C, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P06956
- Elsewhere: RCSB PDB 1PVP · PDBe 1PVP · UniProt P06956
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