Crystal structure of postcleavge complex of escherichia COLI RNase III

Structure

x-ray diffraction · 2 distinct chains · 61% helix, 12% strand (DSSP).

Ligands and binding sites

• MG · Magnesium ion · metal · 6 copies · chains A, C, D, B
• K · Potassium ion · metal · 2 copies · chains C, D
• CL · Chloride ion · ion · 7 copies · chains A, C, B, D
• EDO · 1,2-ethanediol · additive · 14 copies · chains A, D, B, C
• TRS · 2-amino-2-hydroxymethyl-propane-1,3-diol · additive · 2 copies · chains C, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

5 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.

Interfaces

6 interfaces between chains.

• A–B · 1178 Ų buried · 8 hydrogen bonds · protein-protein
• A–C · 916 Ų buried · 28 hydrogen bonds · protein-nucleic
• B–D · 912 Ų buried · 28 hydrogen bonds · protein-nucleic
• A–D · 744 Ų buried · 22 hydrogen bonds · protein-nucleic
• B–C · 733 Ų buried · 21 hydrogen bonds · protein-nucleic

Compared structures

• 7R97 · Crystal structure of postcleavge complex of Escherichia coli RNase III · the same entry in another collection · chain A against A, TM-score 1.00
• P0A7Y0 · AlphaFold monomer V2.0 prediction for ribonuclease 3 (P0A7Y0) · a predicted model of the same protein · chain A against A, TM-score 0.88

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P0A7Y0
• Elsewhere: RCSB PDB 7R97 · PDBe 7R97 · UniProt P0A7Y0