Crystal structure of the ES2 intermediate form of human hydroxymethylbilane synthase

Structure

x-ray diffraction · 2 distinct chains · 38% helix, 23% strand (DSSP).

Ligands and binding sites

• 7J8 · 3-[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-3-(3-hydroxy-3-oxopropyl)-5-methyl-1~{H}-pyrrol-2-yl]methyl]-3-(3-hydroxy-3-oxopropyl)-1~{H}-pyrrol-2-yl]methyl]-3-(3-hydroxy-3-oxopropyl)-1~{H}-pyrrol-2-yl]methyl]-1~{H}-pyrrol-3-yl]propanoic acid · ligand · 2 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

3 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 970 Ų buried · 12 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 2 covalent (covalent ligand).

Compared structures

• 7CCZ · Crystal structure of the ES2 intermediate form of human hydroxymethylbilane synthase · the same entry in another collection · chain A against A, TM-score 1.00
• P08397 · AlphaFold monomer V2.0 prediction for porphobilinogen deaminase (P08397) · a predicted model of the same protein · chain B against A, TM-score 0.97

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P08397
• Elsewhere: RCSB PDB 7CCZ · PDBe 7CCZ · UniProt P08397