Research workbench
Structure of the escherichia COLI clc chloride channel E148A mutant and Fab complex
Analyse Structure of the escherichia COLI clc chloride channel E148A mutant and Fab… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1OTT
- Type
- protein
- Length
- 1,749 residues
- Chains
- 6
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Voltage-gated clc-type chloride channel eric; Fab fragment (heavy chain); Fab fragment…
- UniProt
- P37019
- Ligands
- CL
- Pockets
- 16 · best druggability 0.83
Structure
x-ray diffraction · 4 distinct chains · 49% helix, 16% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Voltage-gated clc-type chloride channel eric | protein | 444 | 72% helix, 0% strand | P37019 |
| B | Voltage-gated clc-type chloride channel eric | protein | 441 | 70% helix, 0% strand | P37019 |
| C, E | Fab fragment (heavy chain) | protein | 221 | 4% helix, 50% strand | — |
| D, F | Fab fragment (light chain) | protein | 211 | 4% helix, 46% strand | — |
Ligands and binding sites
- CL · Chloride ion · ion · 6 copies · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
16 pockets found on a 1 Å grid, 10 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 651 | 0.83 | 0.87 | 60 | empty |
| 2 | 1079 | 0.79 | 0.81 | 99 | empty |
| 3 | 293 | 0.66 | 0.82 | 33 | CL |
| 4 | 279 | 0.64 | 0.81 | 29 | CL |
| 5 | 524 | 0.61 | 0.75 | 56 | empty |
Interfaces
11 interfaces between chains.
- A–B · 3519 Ų buried · 45 hydrogen bonds · protein-protein
- C–D · 1909 Ų buried · 18 hydrogen bonds · protein-protein
- E–F · 1897 Ų buried · 19 hydrogen bonds · protein-protein
- B–E · 488 Ų buried · 7 hydrogen bonds · protein-protein
- A–C · 478 Ų buried · 7 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 8 disulfide (disulfide).
Compared structures
- 1OTT · Structure of the Escherichia coli ClC Chloride channel E148A mutant and Fab Complex · the same entry in another collection · chain F against F, TM-score 1.00
- P37019 · AlphaFold monomer V2.0 prediction for H(+)/CL(-) exchange transporter clca (P37019) · a predicted model of the same protein · chain B against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P37019
- Elsewhere: RCSB PDB 1OTT · PDBe 1OTT · UniProt P37019
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.