Research workbench
Structure of the escherichia COLI clc chloride channel and Fab complex
Analyse Structure of the escherichia COLI clc chloride channel and Fab complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1OTS
- Type
- protein
- Length
- 1,749 residues
- Chains
- 6
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Voltage-gated clc-type chloride channel eric; Fab fragment (heavy chain); Fab fragment…
- UniProt
- P37019
- Ligands
- CL
- Pockets
- 16 · best druggability 0.82
Structure
x-ray diffraction · 4 distinct chains · 53% helix, 17% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Voltage-gated clc-type chloride channel eric | protein | 444 | 77% helix, 0% strand | P37019 |
| B | Voltage-gated clc-type chloride channel eric | protein | 441 | 76% helix, 0% strand | P37019 |
| C, E | Fab fragment (heavy chain) | protein | 221 | 4% helix, 53% strand | — |
| D, F | Fab fragment (light chain) | protein | 211 | 6% helix, 50% strand | — |
Ligands and binding sites
- CL · Chloride ion · ion · 4 copies · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
16 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 565 | 0.82 | 0.88 | 56 | empty |
| 2 | 1024 | 0.81 | 0.83 | 108 | empty |
| 3 | 331 | 0.68 | 0.82 | 32 | CL |
| 4 | 313 | 0.67 | 0.82 | 32 | CL |
| 5 | 603 | 0.64 | 0.75 | 59 | empty |
Interfaces
11 interfaces between chains.
- A–B · 3574 Ų buried · 41 hydrogen bonds · protein-protein
- E–F · 1934 Ų buried · 20 hydrogen bonds · protein-protein
- C–D · 1919 Ų buried · 19 hydrogen bonds · protein-protein
- B–E · 500 Ų buried · 6 hydrogen bonds · protein-protein
- A–C · 478 Ų buried · 5 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 8 disulfide (disulfide).
Compared structures
- 1OTS · Structure of the Escherichia coli ClC Chloride channel and Fab Complex · the same entry in another collection · chain F against F, TM-score 1.00
- P37019 · AlphaFold monomer V2.0 prediction for H(+)/CL(-) exchange transporter clca (P37019) · a predicted model of the same protein · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P37019
- Elsewhere: RCSB PDB 1OTS · PDBe 1OTS · UniProt P37019
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