Research workbench
Crystal structure of two-domain laccase mutant I170A from streptomyces griseoflavus
Analyse Crystal structure of two-domain laccase mutant I170A from streptomyces… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6RHQ
- Type
- protein
- Length
- 1,671 residues
- Chains
- 6
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Two-domain laccase
- EC
- 1.10.3.2
- UniProt
- A0A0M4FJ81
- Ligands
- CU, SO4
- Pockets
- 18 · best druggability 0.88
Structure
x-ray diffraction · 4 distinct chains · 6% helix, 42% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, E | Two-domain laccase (EC 1.10.3.2) | protein | 282 | 6% helix, 42% strand | A0A0M4FJ81 |
| B, D | Two-domain laccase (EC 1.10.3.2) | protein | 278 | 5% helix, 42% strand | A0A0M4FJ81 |
| C | Two-domain laccase (EC 1.10.3.2) | protein | 275 | 5% helix, 42% strand | A0A0M4FJ81 |
| F | Two-domain laccase (EC 1.10.3.2) | protein | 276 | 5% helix, 42% strand | A0A0M4FJ81 |
Ligands and binding sites
- CU · Copper (II) ion · metal · 24 copies · chains A, C, B, D, F, E
- SO4 · Sulfate ion · ion · 2 copies · chains E, D, F
- GOL · Glycerol · additive · 2 copies · chains D, E
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
18 pockets found on a 1 Å grid, 16 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1022 | 0.88 | 0.90 | 124 | empty |
| 2 | 373 | 0.84 | 0.93 | 54 | empty |
| 3 | 359 | 0.83 | 0.92 | 47 | empty |
| 4 | 269 | 0.75 | 0.89 | 39 | empty |
| 5 | 51 | 0.71 | 0.99 | 19 | empty |
Interfaces
14 interfaces between chains.
- D–E · 1881 Ų buried · 41 hydrogen bonds · protein-protein
- A–C · 1867 Ų buried · 36 hydrogen bonds · protein-protein
- A–B · 1832 Ų buried · 35 hydrogen bonds · protein-protein
- E–F · 1795 Ų buried · 35 hydrogen bonds · protein-protein
- D–F · 1787 Ų buried · 35 hydrogen bonds · protein-protein
Compared structures
- 6RHQ · Crystal Structure of Two-Domain Laccase mutant I170A from Streptomyces griseoflavus · the same entry in another collection · chain B against B, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt A0A0M4FJ81
- Elsewhere: RCSB PDB 6RHQ · PDBe 6RHQ · UniProt A0A0M4FJ81
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.