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Crystal structure of bacillus subtilis sigw domain 4 in complexed with -35 element DNA

Analyse Crystal structure of bacillus subtilis sigw domain 4 in complexed with -35… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB
6JHE
Type
protein + DNA
Length
75 residues
Chains
3
Method
x-ray diffraction
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule
Ecf RNA polymerase sigma factor sigw; DNA (5'-D(P*TP*TP*GP*AP*AP*AP*CP*CP*TP*TP*T)-3')…
UniProt
Q45585

Structure

x-ray diffraction · 3 distinct chains · 70% helix, 0% strand (DSSP).

ChainMoleculeTypeResiduesSecondary structureUniProt
AEcf RNA polymerase sigma factor sigwprotein5370% helix, 0% strandQ45585
BDNA (5'-D(P*TP*TP*GP*AP*AP*AP*CP*CP*TP*TP*T)-3')dna11——
CDNA (5'-D(*AP*AP*AP*GP*GP*TP*TP*TP*CP*AP*A)-3')dna11——

Interfaces

3 interfaces between chains.

  • B–C · 465 Ų buried · 41 hydrogen bonds · nucleic-nucleic
  • A–C · 402 Ų buried · 4 hydrogen bonds · protein-nucleic
  • A–B · 327 Ų buried · 7 hydrogen bonds · protein-nucleic

Compared structures

  • 6JHE · Crystal Structure of Bacillus subtilis SigW domain 4 in complexed with -35 element DNA · the same entry in another collection · chain A against A, TM-score 1.00
  • Q45585 · AlphaFold monomer V2.0 prediction for ecf RNA polymerase sigma factor sigw (Q45585) · a predicted model of the same protein · chain A against A, TM-score 0.87

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