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Crystal structure of chaetomium thermophilum BRR2 helicase core in complex with PRP8 JAB1 domain

Analyse Crystal structure of chaetomium thermophilum BRR2 helicase core in complex with… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 5M59
Type: protein
Length: 7,951 residues
Chains: 8
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Putative pre-mRNA splicing factor; Pre-mRNA splicing helicase-like protein
UniProt: G0SFL3, G0S0B9
Pockets: 20 · best druggability 0.89

Structure

x-ray diffraction · 8 distinct chains · 37% helix, 16% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
B	Putative pre-mRNA splicing factor	protein	266	23% helix, 26% strand	G0SFL3
A	Pre-mRNA splicing helicase-like protein	protein	1738	41% helix, 16% strand	G0S0B9
D	Putative pre-mRNA splicing factor	protein	263	22% helix, 27% strand	G0SFL3
C	Pre-mRNA splicing helicase-like protein	protein	1702	40% helix, 13% strand	G0S0B9
F	Putative pre-mRNA splicing factor	protein	265	23% helix, 27% strand	G0SFL3
E	Pre-mRNA splicing helicase-like protein	protein	1740	39% helix, 16% strand	G0S0B9
H	Putative pre-mRNA splicing factor	protein	267	25% helix, 25% strand	G0SFL3
G	Pre-mRNA splicing helicase-like protein	protein	1710	38% helix, 15% strand	G0S0B9

Ligands and binding sites

ACT · Acetate ion · additive · 4 copies · chains A, C, E, G

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1035	0.89	0.90	102	empty
2	1023	0.89	0.90	117	empty
3	803	0.88	0.90	99	empty
4	950	0.87	0.88	91	empty
5	1157	0.85	0.86	91	empty

Interfaces

10 interfaces between chains.

B–A · 2616 Å² buried · 38 hydrogen bonds · protein-protein
H–G · 2615 Å² buried · 33 hydrogen bonds · protein-protein
D–C · 2593 Å² buried · 32 hydrogen bonds · protein-protein
F–E · 2563 Å² buried · 31 hydrogen bonds · protein-protein
F–G · 547 Å² buried · 3 hydrogen bonds · protein-protein

Compared structures

5M59 · Crystal structure of Chaetomium thermophilum Brr2 helicase core in complex with Prp8 Jab1… · the same entry in another collection · chain B against B, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt G0SFL3
Every structure of UniProt G0S0B9
Elsewhere: RCSB PDB 5M59 · PDBe 5M59 · UniProt G0SFL3 · UniProt G0S0B9

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