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Structural determination of an protein complex of a Fab with increased solubility
Analyse Structural determination of an protein complex of a Fab with increased… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5EII
- Type
- protein
- Length
- 1,129 residues
- Chains
- 6
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Fab heavy chain; Fab light chain; Histone chaperone ASF1
- UniProt
- P32447
- Pockets
- 14 · best druggability 0.73
Structure
x-ray diffraction · 5 distinct chains · 6% helix, 53% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Fab heavy chain | protein | 220 | 8% helix, 54% strand | — |
| B, L | Fab light chain | protein | 212 | 6% helix, 51% strand | — |
| G | Histone chaperone ASF1 | protein | 144 | 8% helix, 53% strand | P32447 |
| H | Fab heavy chain | protein | 225 | 8% helix, 53% strand | — |
| I | Histone chaperone ASF1 | protein | 116 | 0% helix, 53% strand | P32447 |
Pockets
14 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1089 | 0.73 | 0.76 | 79 | empty |
| 2 | 143 | 0.58 | 0.83 | 21 | empty |
| 3 | 176 | 0.57 | 0.80 | 20 | empty |
| 4 | 60 | 0.54 | 0.88 | 22 | empty |
| 5 | 297 | 0.54 | 0.75 | 38 | empty |
Interfaces
10 interfaces between chains.
- H–L · 1890 Ų buried · 11 hydrogen bonds · protein-protein
- A–B · 1726 Ų buried · 12 hydrogen bonds · protein-protein
- G–H · 643 Ų buried · 13 hydrogen bonds · protein-protein
- A–I · 621 Ų buried · 17 hydrogen bonds · protein-protein
- G–I · 289 Ų buried · 2 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 8 disulfide (disulfide).
Compared structures
- 5EII · Structural determination of an protein complex of a Fab with increased solubility · the same entry in another collection · chain L against L, TM-score 1.00
- P32447 · AlphaFold monomer V2.0 prediction for histone chaperone ASF1 (P32447) · a predicted model of the same protein · chain G against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P32447
- Elsewhere: RCSB PDB 5EII · PDBe 5EII · UniProt P32447
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