Research workbench
Constitutively active sin recombinase cataltyic domain - I100T
Analyse Constitutively active sin recombinase cataltyic domain - I100T in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5C32
- Type
- protein
- Length
- 492 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Putative transposon TN552 DNA-invertase BIN3
- UniProt
- P20384
- Ligands
- SO4
- Pockets
- 7 · best druggability 0.69
Structure
x-ray diffraction · 3 distinct chains · 47% helix, 16% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| B, C | Putative transposon TN552 DNA-invertase BIN3 | protein | 126 | 46% helix, 17% strand | P20384 |
| D | Putative transposon TN552 DNA-invertase BIN3 | protein | 122 | 48% helix, 14% strand | P20384 |
| A | Putative transposon TN552 DNA-invertase BIN3 | protein | 118 | 48% helix, 18% strand | P20384 |
Ligands and binding sites
- SO4 · Sulfate ion · ion · 4 copies · chains B, D, A, C
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
7 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 391 | 0.69 | 0.82 | 48 | empty |
| 2 | 258 | 0.62 | 0.80 | 31 | empty |
| 3 | 216 | 0.57 | 0.79 | 25 | empty |
| 4 | 157 | 0.55 | 0.80 | 31 | empty |
| 5 | 175 | 0.51 | 0.78 | 24 | empty |
Interfaces
6 interfaces between chains.
- B–A · 888 Ų buried · 10 hydrogen bonds · protein-protein
- D–C · 802 Ų buried · 8 hydrogen bonds · protein-protein
- B–C · 551 Ų buried · 0 hydrogen bonds · protein-protein
- D–A · 317 Ų buried · 0 hydrogen bonds · protein-protein
- B–D · 203 Ų buried · 0 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 26 modified (engineered).
Compared structures
- 5C32 · Constitutively active Sin recombinase cataltyic domain - I100T · the same entry in another collection · chain C against C, TM-score 1.00
- P20384 · AlphaFold monomer V2.0 prediction for putative transposon TN552 DNA- invertase BIN3… · a predicted model of the same protein · chain A against A, TM-score 0.95
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P20384
- Elsewhere: RCSB PDB 5C32 · PDBe 5C32 · UniProt P20384
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.