Research workbench

Crystal structure of the receiver domain of cpxr from E. COLI (phosphorylated)

Analyse Crystal structure of the receiver domain of cpxr from E. COLI (phosphorylated) in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 4UHK
Type: protein
Length: 386 residues
Chains: 3
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Transcriptional regulatory protein cpxr
UniProt: P0AE88
Ligands: MG
Pockets: 4 · best druggability 0.56

Structure

x-ray diffraction · 2 distinct chains · 49% helix, 18% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Transcriptional regulatory protein cpxr	protein	130	48% helix, 18% strand	P0AE88
B, C	Transcriptional regulatory protein cpxr	protein	128	49% helix, 18% strand	P0AE88

Ligands and binding sites

MG · Magnesium ion · metal · 3 copies · chains A, B, C

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

4 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	256	0.56	0.77	29	empty
2	165	0.55	0.80	21	empty
3	116	0.48	0.80	19	empty
4	105	0.39	0.77	17	empty

Interfaces

2 interfaces between chains.

B–C · 1468 Å² buried · 29 hydrogen bonds · protein-protein
A–B · 231 Å² buried · 6 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 3 modified (phosphorylation).

Compared structures

4UHK · Crystal structure of the receiver domain of CpxR from E. coli (phosphorylated) · the same entry in another collection · chain A against A, TM-score 1.00
P0AE88 · AlphaFold monomer V2.0 prediction for transcriptional regulatory protein cpxr (P0AE88) · a predicted model of the same protein · chain C against A, TM-score 0.93

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P0AE88
Elsewhere: RCSB PDB 4UHK · PDBe 4UHK · UniProt P0AE88

GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.