Research workbench
Crystal structure of the angiotensin-1 converting enzyme N-domain in complex with lys-pro
Analyse Crystal structure of the angiotensin-1 converting enzyme N-domain in complex… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 4UFB
- Type
- protein
- Length
- 2,432 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Angiotensin-converting enzyme
- EC
- 3.4.15.1
- UniProt
- P12821
- Ligands
- NAG, FUC, BMA, ZN, CL
- Pockets
- 20 · best druggability 0.84
Structure
x-ray diffraction · 4 distinct chains · 62% helix, 3% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Angiotensin-converting enzyme (EC 3.4.15.1) | protein | 605 | 62% helix, 3% strand | P12821 |
| B | Angiotensin-converting enzyme (EC 3.4.15.1) | protein | 607 | 62% helix, 2% strand | P12821 |
| C | Angiotensin-converting enzyme (EC 3.4.15.1) | protein | 609 | 62% helix, 3% strand | P12821 |
| D | Angiotensin-converting enzyme (EC 3.4.15.1) | protein | 611 | 62% helix, 3% strand | P12821 |
Ligands and binding sites
- NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 20 copies · chains A, B, D, C
- FUC · alpha-L-fucopyranose · sugar · 6 copies · chains A, B, C, D
- BMA · beta-D-mannopyranose · sugar · 4 copies · chains A, B, C, D
- ZN · Zinc ion · metal · 4 copies · chains A, B, C, D
- CL · Chloride ion · ion · 4 copies · chains A, B, C, D
- PEG · Di(hydroxyethyl)ether · additive · 10 copies · chains A, C, B, D
- P6G · Hexaethylene glycol · additive · 6 copies · chains A, C, B, D
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 14 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 854 | 0.84 | 0.86 | 76 | PEG |
| 2 | 1191 | 0.83 | 0.84 | 77 | P6G |
| 3 | 753 | 0.82 | 0.86 | 69 | PEG, P6G |
| 4 | 758 | 0.82 | 0.86 | 69 | P6G |
| 5 | 372 | 0.82 | 0.91 | 62 | empty |
Interfaces
3 interfaces between chains.
- B–D · 1061 Ų buried · 14 hydrogen bonds · protein-protein
- A–C · 293 Ų buried · 2 hydrogen bonds · protein-protein
- A–D · 144 Ų buried · 0 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 12 disulfide, 12 glycan (disulfide, N-glycosylation).
Compared structures
- 4UFB · Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with Lys-Pro · the same entry in another collection · chain D against D, TM-score 1.00
- P12821 · AlphaFold monomer V2.0 prediction for angiotensin-converting enzyme (P12821) · a predicted model of the same protein · chain A against A, TM-score 0.99
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P12821
- Elsewhere: RCSB PDB 4UFB · PDBe 4UFB · UniProt P12821
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