Research workbench
Structure of Anisomycin resistant 50S Ribosomal Subunit: 23S rRNA mutation U2535A
Analyse Structure of Anisomycin resistant 50S Ribosomal Subunit: 23S rRNA mutation… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3CCE
- Type
- protein + RNA
- Length
- 6,651 residues
- Chains
- 31
- Method
- x-ray diffraction
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 2.75
- Source
- Haloarcula marismortui
- Molecule
- 50S ribosomal protein L2P; 50S ribosomal protein L3P; 50S ribosomal protein L4P; 50S…
- UniProt
- P20276, P20279, P12735, P14124, P14135, P12743, P15825, P60617, P14122, P29198, P22450, P12737, P60618, P14123, P12733, P14119, P12734, P10970, P12732, P10972, P14116, P10971, P14121, P18138, P12736, P32410, P22452, P32411
- Ligands
- SR, MG, NA, CD, K, CL
- Pockets
- 20 · best druggability 0.87
Structure
x-ray diffraction · resolution 2.75 · 31 distinct chains · 30% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | 50S ribosomal protein L2P | protein | 237 | 12% helix, 25% strand | P20276 |
| B | 50S ribosomal protein L3P | protein | 337 | 14% helix, 32% strand | P20279 |
| C | 50S ribosomal protein L4P | protein | 246 | 32% helix, 14% strand | P12735 |
| D | 50S ribosomal protein L5P | protein | 140 | 24% helix, 24% strand | P14124 |
| E | 50S ribosomal protein L6P | protein | 172 | 23% helix, 39% strand | P14135 |
| F | 50S ribosomal protein L7Ae | protein | 119 | 49% helix, 15% strand | P12743 |
| G | 50S ribosomal protein L10E | protein | 29 | 79% helix, 0% strand | P15825 |
| H | 50S ribosomal protein L10e | protein | 160 | 24% helix, 25% strand | P60617 |
| I | 50S ribosomal protein L11P | protein | 70 | 44% helix, 6% strand | P14122 |
| J | 50S ribosomal protein L13P | protein | 142 | 38% helix, 15% strand | P29198 |
| K | 50S ribosomal protein L14P | protein | 132 | 8% helix, 36% strand | P22450 |
| L | 50S ribosomal protein L15P | protein | 145 | 20% helix, 16% strand | P12737 |
| M | 50S ribosomal protein L15e | protein | 194 | 33% helix, 16% strand | P60618 |
| N | 50S ribosomal protein L18P | protein | 186 | 39% helix, 13% strand | P14123 |
| O | 50S ribosomal protein L18e | protein | 115 | 45% helix, 23% strand | P12733 |
| P | 50S ribosomal protein L19e | protein | 143 | 60% helix, 4% strand | P14119 |
| Q | 50S ribosomal protein L21e | protein | 95 | 14% helix, 32% strand | P12734 |
| R | 50S ribosomal protein L22P | protein | 150 | 32% helix, 31% strand | P10970 |
| S | 50S ribosomal protein L23P | protein | 81 | 33% helix, 31% strand | P12732 |
| T | 50S ribosomal protein L24P | protein | 119 | 29% helix, 29% strand | P10972 |
| U | 50S ribosomal protein L24e | protein | 53 | 21% helix, 19% strand | P14116 |
| V | 50S ribosomal protein L29P | protein | 65 | 74% helix, 0% strand | P10971 |
| W | 50S ribosomal protein L30P | protein | 154 | 40% helix, 14% strand | P14121 |
| X | 50S ribosomal protein L31e | protein | 82 | 43% helix, 23% strand | P18138 |
| Y | 50S ribosomal protein L32e | protein | 142 | 23% helix, 12% strand | P12736 |
| Z | 50S ribosomal protein L37Ae | protein | 73 | 34% helix, 22% strand | — |
| 1 | 50S ribosomal protein L37e | protein | 56 | 13% helix, 16% strand | P32410 |
| 2 | 50S ribosomal protein L39e | protein | 46 | 43% helix, 0% strand | P22452 |
| 3 | 50S ribosomal protein L44E | protein | 92 | 14% helix, 32% strand | P32411 |
| 0 | 23S ribosomal RNA | rna | 2754 | — | — |
| 9 | 5S ribosomal RNA | rna | 122 | — | — |
Ligands and binding sites
- SR · Strontium ion · metal · 108 copies · chains A, 0, B, F, R, S, 1, 3, H, M, T, L, Y, J, 9
- MG · Magnesium ion · metal · 93 copies · chains A, 0, B, K, T, Y, C, 1, S, R, 9, E, W, 2, 3, P, M
- NA · Sodium ion · metal · 75 copies · chains C, J, 0, M, Q, R, S, B, 2, H, Y, T, 9, L, D
- CD · Cadmium ion · metal · 5 copies · chains O, U, Z, 1, 3
- K · Potassium ion · metal · 2 copies · chains 0, M
- CL · Chloride ion · ion · 22 copies · chains A, B, 0, E, J, L, M, D, N, O, Q, R, 3, 2, K, Y, W
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1182 | 0.87 | 0.90 | 111 | MG, NA, CL, SR |
| 2 | 695 | 0.87 | 0.91 | 88 | CL, MG, SR |
| 3 | 796 | 0.86 | 0.90 | 74 | empty |
| 4 | 1150 | 0.86 | 0.88 | 110 | SR |
| 5 | 720 | 0.86 | 0.90 | 84 | empty |
Interfaces
56 interfaces between chains.
- B–0 · 7389 Ų buried · 216 hydrogen bonds · protein-nucleic
- M–0 · 6539 Ų buried · 177 hydrogen bonds · protein-nucleic
- A–0 · 6294 Ų buried · 177 hydrogen bonds · protein-nucleic
- C–0 · 5575 Ų buried · 143 hydrogen bonds · protein-nucleic
- P–0 · 4367 Ų buried · 128 hydrogen bonds · protein-nucleic
Modifications
Observed in the structure: 5 modified (nucleotide modification).
Compared structures
- 3CCE · Structure of anisomycin resistant 50S ribosomal subunit: 23S rRNA mutation U2535A · the same entry in another collection · chain E against E, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P20276
- Every structure of UniProt P20279
- Every structure of UniProt P12735
- Every structure of UniProt P14124
- Every structure of UniProt P14135
- Every structure of UniProt P12743
- Every structure of UniProt P15825
- Every structure of UniProt P60617
- Every structure of UniProt P14122
- Every structure of UniProt P29198
- Every structure of UniProt P22450
- Every structure of UniProt P12737
- Every structure of UniProt P60618
- Every structure of UniProt P14123
- Every structure of UniProt P12733
- Every structure of UniProt P14119
- Every structure of UniProt P12734
- Every structure of UniProt P10970
- Every structure of UniProt P12732
- Every structure of UniProt P10972
- Every structure of UniProt P14116
- Every structure of UniProt P10971
- Every structure of UniProt P14121
- Every structure of UniProt P18138
- Every structure of UniProt P12736
- Every structure of UniProt P32410
- Every structure of UniProt P22452
- Every structure of UniProt P32411
- Elsewhere: RCSB PDB 3CCE · PDBe 3CCE · UniProt P20276 · UniProt P20279 · UniProt P12735 · UniProt P14124 · UniProt P14135 · UniProt P12743 · UniProt P15825 · UniProt P60617 · UniProt P14122 · UniProt P29198 · UniProt P22450 · UniProt P12737 · UniProt P60618 · UniProt P14123 · UniProt P12733 · UniProt P14119 · UniProt P12734 · UniProt P10970 · UniProt P12732 · UniProt P10972 · UniProt P14116 · UniProt P10971 · UniProt P14121 · UniProt P18138 · UniProt P12736 · UniProt P32410 · UniProt P22452 · UniProt P32411
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