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8WLP: Cryo-EM structure of the distal rod-hook within the flagellar motor-hook complex in the CCW state.

Analyse 8WLP: Cryo-EM structure of the distal rod-hook within the flagellar motor-hook… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 8WLP
Type: protein
Length: 17,770 residues
Chains: 53
Method: electron microscopy
Authors: Tan, J.X., Zhang, L., Zhou, Y., Zhu, Y.Q.
Deposited: 09/30/23
Collection: MolNFT v2 / PDB Bank
Contract: 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
Owner: 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
Resolution: 3.8
Source: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Molecule: Flagellar basal-body rod protein FlgG; Flagellar hook protein FlgE
UniProt: P0A1J3, P0A1J1
Pockets: 20 · best druggability 0.88

Structure

electron microscopy · resolution 3.8 · 8 distinct chains · 23% helix, 33% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
0, x, y, z	Flagellar basal-body rod protein FlgG	protein	248	24% helix, 33% strand	P0A1J3
1	Flagellar basal-body rod protein FlgG	protein	252	24% helix, 33% strand	P0A1J3
2, 3, 4, 5, 6, 7, 8, 9, ZA, ZB, ZC, ZD, ZE	Flagellar basal-body rod protein FlgG	protein	260	25% helix, 28% strand	P0A1J3
ZF, ZG, ZH, ZI, ZJ, ZK, ZL, ZM, ZN, ZO, ZP, ZQ, ZR, ZS, ZT, ZU, ZV, ZW, ZX, ZY, ZZ, Za, Zb, Zc, Zd, Ze, Zf, Zg, Zh	Flagellar hook protein FlgE	protein	401	15% helix, 40% strand	P0A1J1
r, u	Flagellar basal-body rod protein FlgG	protein	254	25% helix, 30% strand	P0A1J3
s, v	Flagellar basal-body rod protein FlgG	protein	255	24% helix, 29% strand	P0A1J3
t	Flagellar basal-body rod protein FlgG	protein	256	25% helix, 33% strand	P0A1J3
w	Flagellar basal-body rod protein FlgG	protein	243	24% helix, 31% strand	P0A1J3

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	875	0.88	0.91	97	empty
2	901	0.88	0.90	101	empty
3	882	0.88	0.91	107	empty
4	894	0.88	0.90	125	empty
5	912	0.88	0.90	105	empty

Interfaces

194 interfaces between chains.

5–ZA · 2747 Å² buried · 33 hydrogen bonds · protein-protein
1–w · 2738 Å² buried · 27 hydrogen bonds · protein-protein
2–7 · 2724 Å² buried · 31 hydrogen bonds · protein-protein
2–x · 2717 Å² buried · 29 hydrogen bonds · protein-protein
3–y · 2654 Å² buried · 21 hydrogen bonds · protein-protein

Compared structures

P0A1J3 · AlphaFold monomer V2.0 prediction for flagellar basal-body rod protein flgg (P0A1J3) · a predicted model of the same protein · chain ZA against A, TM-score 0.97

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P0A1J3
Every structure of UniProt P0A1J1
Elsewhere: RCSB PDB 8WLP · PDBe 8WLP · UniProt P0A1J3 · UniProt P0A1J1

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