Research workbench
V. cholerae TniQ-Cascade complex with Type III-B crRNA
Analyse V. cholerae TniQ-Cascade complex with Type III-B crRNA in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 8FUK
- Type
- protein + RNA
- Length
- 2,844 residues
- Chains
- 11
- Method
- electron microscopy
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 3.36
- Source
- metagenome; Vibrio cholerae
- Molecule
- Type III-B crRNA; Cas7; CRISPR-associated protein Cas8; Cas6; TniQ
- UniProt
- A0A6I8WFX5, A0A6I8WFX4, A0A6I8WFX3, A0A6I8WFX7
- Pockets
- 20 · best druggability 0.81
Structure
electron microscopy · resolution 3.36 · 11 distinct chains · 31% helix, 23% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| 1 | Type III-B crRNA | rna | 60 | — | — |
| A | Cas7 | protein | 329 | 29% helix, 26% strand | A0A6I8WFX5 |
| B | Cas7 | protein | 337 | 31% helix, 20% strand | A0A6I8WFX5 |
| C | Cas7 | protein | 328 | 31% helix, 29% strand | A0A6I8WFX5 |
| D | Cas7 | protein | 336 | 29% helix, 25% strand | A0A6I8WFX5 |
| E | Cas7 | protein | 333 | 31% helix, 24% strand | A0A6I8WFX5 |
| F | Cas7 | protein | 294 | 32% helix, 25% strand | A0A6I8WFX5 |
| G | CRISPR-associated protein Cas8 | protein | 339 | 19% helix, 31% strand | A0A6I8WFX4 |
| H | Cas6 | protein | 197 | 26% helix, 20% strand | A0A6I8WFX3 |
| I | TniQ | protein | 182 | 57% helix, 3% strand | A0A6I8WFX7 |
| J | TniQ | protein | 109 | 40% helix, 8% strand | A0A6I8WFX7 |
Pockets
20 pockets found on a 1 Å grid, 19 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1054 | 0.81 | 0.82 | 86 | empty |
| 2 | 144 | 0.80 | 0.97 | 39 | empty |
| 3 | 99 | 0.77 | 0.98 | 41 | empty |
| 4 | 823 | 0.75 | 0.79 | 76 | empty |
| 5 | 730 | 0.74 | 0.80 | 74 | empty |
Interfaces
26 interfaces between chains.
- D–E · 2244 Ų buried · 21 hydrogen bonds · protein-protein
- B–C · 2235 Ų buried · 18 hydrogen bonds · protein-protein
- A–B · 2232 Ų buried · 23 hydrogen bonds · protein-protein
- E–F · 2206 Ų buried · 22 hydrogen bonds · protein-protein
- C–D · 2120 Ų buried · 26 hydrogen bonds · protein-protein
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt A0A6I8WFX5
- Every structure of UniProt A0A6I8WFX4
- Every structure of UniProt A0A6I8WFX3
- Every structure of UniProt A0A6I8WFX7
- Elsewhere: RCSB PDB 8FUK · PDBe 8FUK · UniProt A0A6I8WFX5 · UniProt A0A6I8WFX4 · UniProt A0A6I8WFX3 · UniProt A0A6I8WFX7
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.