Research workbench
Cascade complex from type I-A CRISPR-Cas system
Analyse Cascade complex from type I-A CRISPR-Cas system in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7TR6
- Type
- protein + RNA
- Length
- 3,428 residues
- Chains
- 15
- Method
- electron microscopy
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 3.4
- Source
- Escherichia coli; Pyrococcus furiosus DSM 3638
- Molecule
- Cas8a; Cas11a; Cas7a; Cas5a; crRNA (45-MER)
- UniProt
- Q8U338, Q8U332, Q8U333, A0A5C0XNV9
- Pockets
- 20 · best druggability 0.90
Structure
electron microscopy · resolution 3.4 · 9 distinct chains · 32% helix, 22% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| C | Cas8a | protein | 333 | 46% helix, 13% strand | Q8U338 |
| D, F, G, H | Cas11a | protein | 107 | 60% helix, 0% strand | Q8U332 |
| E | Cas11a | protein | 108 | 64% helix, 0% strand | Q8U332 |
| I | Cas7a | protein | 335 | 23% helix, 28% strand | Q8U333 |
| J, K, L, M | Cas7a | protein | 336 | 26% helix, 27% strand | Q8U333 |
| N | Cas7a | protein | 321 | 25% helix, 28% strand | Q8U333 |
| O | Cas7a | protein | 274 | 27% helix, 24% strand | Q8U333 |
| P | Cas5a | protein | 240 | 18% helix, 26% strand | A0A5C0XNV9 |
| R | crRNA (45-MER) | rna | 45 | — | — |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 941 | 0.90 | 0.92 | 151 | empty |
| 2 | 974 | 0.85 | 0.87 | 103 | empty |
| 3 | 397 | 0.84 | 0.92 | 70 | empty |
| 4 | 713 | 0.83 | 0.87 | 104 | empty |
| 5 | 734 | 0.83 | 0.87 | 70 | empty |
Interfaces
46 interfaces between chains.
- M–N · 2494 Ų buried · 22 hydrogen bonds · protein-protein
- L–M · 2478 Ų buried · 31 hydrogen bonds · protein-protein
- J–K · 2454 Ų buried · 25 hydrogen bonds · protein-protein
- N–O · 2450 Ų buried · 22 hydrogen bonds · protein-protein
- I–J · 2438 Ų buried · 14 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 5 disulfide (disulfide).
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q8U338
- Every structure of UniProt Q8U332
- Every structure of UniProt Q8U333
- Every structure of UniProt A0A5C0XNV9
- Elsewhere: RCSB PDB 7TR6 · PDBe 7TR6 · UniProt Q8U338 · UniProt Q8U332 · UniProt Q8U333 · UniProt A0A5C0XNV9
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