Crystal structure of murine 4-1BB N128A mutant from HEK293T cells in P43 space group

Structure

x-ray diffraction · resolution 2.3 · 2 distinct chains · 1% helix, 34% strand (DSSP).

Ligands and binding sites

• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 3 copies · chains B, A
• BMA · beta-D-mannopyranose · sugar
• MAN · alpha-D-mannopyranose · sugar
• FUC · alpha-L-fucopyranose · sugar · chains B
• SO4 · Sulfate ion · ion · 3 copies · chains A, B
• GOL · Glycerol · additive · 2 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

1 interface between chains.

• A–B · 24 Ų buried · 0 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 16 disulfide, 2 glycan (disulfide, N-glycosylation).

Compared structures

• 5WIW · Crystal structure of murine 4-1BB N128A mutant from HEK293T cells in P43 space group · the same entry in another collection · chain A against A, TM-score 1.00
• P20334 · AlphaFold monomer V2.0 prediction for tumor necrosis factor receptor superfamily member 9… · a predicted model of the same protein · chain B against A, TM-score 0.92

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P20334
• Elsewhere: RCSB PDB 5WIW · PDBe 5WIW · UniProt P20334