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MolNFT v2 · #203621 · protein

Structure of yeast oligosaccharylransferase complex with lipid-linked oligosaccharide and non-acceptor peptide bound

8AGC: “Structure of yeast oligosaccharylransferase complex with lipid-linked oligosaccharide and non-acceptor…”. Electron microscopy at 3.1, 10 ligands (CPL, PTY, ELU). MolNFT v2 #203621 on GenesisL1: 3D structure, binding sites and…

PDB
8AGC
Type
protein
Length
2,151 residues
Chains
9
Method
electron microscopy
Collection
MolNFT v2 / PDB Bank
Contract
0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
Owner
0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
Resolution
3.1
Source
Saccharomyces cerevisiae; SYNTHETIC CONSTRUCT
UniProt
A0A6A5Q0M3, A0A8H8UM72, Q92316, A0A8H4BUV6, A0A6A5PXA1, A0A6V8S2Y6, A0A8H8ULL1, A0A6A5Q3S9
Ligands
CPL, PTY, ELU, 323, NAG, MAN, GLC, BMA, NDG, MN

Structure

electron microscopy · resolution 3.1 · 9 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
A—protein697—A0A6A5Q0M3
B—protein33—A0A8H8UM72
C—protein85—Q92316
D—protein109—A0A8H4BUV6
E—protein438—A0A6A5PXA1
F—protein258—A0A6V8S2Y6
G—protein394—A0A8H8ULL1
H—protein131—A0A6A5Q3S9
P—protein6——

Ligands and binding sites

  • CPL · 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine · ligand · 4 copies · chains A, B, E, C, D, F, G
  • PTY · Phosphatidylethanolamine · ligand · 2 copies · chains A, F, H
  • ELU · phosphono [(3~{R},6~{E},10~{E})-3,7,11,15-tetramethylhexadeca-6,10,14-trienyl] hydrogen phosphate · ligand · chains A
  • 323 · 2-[3,6-bis(dimethylamino)xanthen-9-yl]-5-methanoyl-benzoate · ligand · chains A, H, P
  • NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 9 copies · chains A, G, E
  • MAN · alpha-D-mannopyranose · sugar · 9 copies · chains F, G, A
  • GLC · alpha-D-glucopyranose · sugar · 3 copies · chains D, G
  • BMA · beta-D-mannopyranose · sugar · 2 copies · chains G, A
  • NDG · 2-acetamido-2-deoxy-alpha-D-glucopyranose · sugar · chains A, P
  • MN · Manganese (II) ion · metal · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • Q92316 · AlphaFold monomer V2.0 prediction for dolichyl- diphosphooligosaccharide--protein… · a predicted model of the same protein · chain C against A, TM-score 0.87

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