Oxime side-chain cross-links in the GCN4-P1 dimeric coiled coil: linear precursor

Structure

x-ray diffraction · 2 distinct chains · 94% helix, 0% strand (DSSP).

Ligands and binding sites

• ACE · Acetyl group · ligand · 2 copies · chains A, B
• ACT · Acetate ion · additive · 3 copies · chains B
• GOL · Glycerol · additive · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

1 interface between chains.

• A–B · 944 Ų buried · 2 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 6 modified, 2 covalent (nonstandard, covalent ligand).

Compared structures

• 4HU5 · Oxime side-chain cross-links in the GCN4-p1 dimeric coiled coil: Linear precursor · the same entry in another collection · chain A against A, TM-score 1.00
• P03069 · AlphaFold monomer V2.0 prediction for general control transcription factor GCN4 (P03069) · a predicted model of the same protein · chain B against A, TM-score 0.97

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P03069
• Elsewhere: RCSB PDB 4HU5 · PDBe 4HU5 · UniProt P03069