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MolNFT v1 · #94318 · protein + RNA

Crystal structure of E COLI hfq bound to two rnas

4HT9 · Protein hfq: “Crystal structure of E COLI hfq bound to two rnas”. X-ray diffraction. MolNFT v1 #94318 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB
4HT9
Type
protein + RNA
Length
191 residues
Chains
5
Method
x-ray diffraction
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule
Protein hfq; RNA (5'-R(*AP*AP*AP*AP*AP*AP*A)-3'); RNA (5'-R(*AP*UP*UP*UP*UP*UP*UP*A)-3')
UniProt
C6ECV6

Structure

x-ray diffraction · 4 distinct chains · 21% helix, 51% strand (DSSP).

ChainMoleculeTypeResiduesSecondary structureUniProt
A, CProtein hfqprotein6022% helix, 52% strandC6ECV6
BProtein hfqprotein6221% helix, 50% strandC6ECV6
DRNA (5'-R(*AP*AP*AP*AP*AP*AP*A)-3')rna7——
ERNA (5'-R(*AP*UP*UP*UP*UP*UP*UP*A)-3')rna2——

Interfaces

8 interfaces between chains.

  • A–B · 680 Ų buried · 10 hydrogen bonds · protein-protein
  • A–C · 666 Ų buried · 9 hydrogen bonds · protein-protein
  • A–D · 425 Ų buried · 8 hydrogen bonds · protein-nucleic
  • B–D · 424 Ų buried · 8 hydrogen bonds · protein-nucleic
  • A–E · 272 Ų buried · 5 hydrogen bonds · protein-nucleic

Compared structures

  • 4HT9 · Crystal structure of E coli Hfq bound to two RNAs · the same entry in another collection · chain A against A, TM-score 1.00

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