Glucoamylase-471 complexed with D-gluco-dihydroacarbose

Structure

x-ray diffraction · 1 distinct chain · 49% helix, 7% strand (DSSP).

Ligands and binding sites

• RY7 · 4,6-dideoxy-4-{[(1S,2S,3S,4R,5R)-2,3,4-trihydroxy-5-(hydroxymethyl)cyclohexyl]amino}-alpha-D-glucopyranose · ligand · chains A
• MAN · alpha-D-mannopyranose · sugar · 18 copies · chains A
• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 4 copies · chains A
• BMA · beta-D-mannopyranose · sugar · 2 copies · chains A
• GLC · alpha-D-glucopyranose · sugar · 2 copies · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

2 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.

Modifications

Observed in the structure: 12 glycan, 3 disulfide (O-glycosylation, disulfide, N-glycosylation).

Compared structures

• 1GAI · Glucoamylase-471 complexed with D-gluco-dihydroacarbose · the same entry in another collection · chain A against A, TM-score 1.00
• P22832 · AlphaFold monomer V2.0 prediction for glucoamylase (P22832) · a predicted model of the same protein · chain A against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P22832
• Elsewhere: RCSB PDB 1GAI · PDBe 1GAI · UniProt P22832