Green-fluorescent antibody 11G10 in complex with its hapten (nitro-stilbene derivative)

Structure

x-ray diffraction · 2 distinct chains · 6% helix, 53% strand (DSSP).

Ligands and binding sites

• TNS · N-(trans-4'-nitro-4-stilbenyl)-N-methyl-5-amino-pentanoic acid · ligand · 2 copies · chains L, H, A, B
• SO4 · Sulfate ion · ion · 3 copies · chains L, H, A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

15 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.

Interfaces

6 interfaces between chains.

• L–H · 1865 Ų buried · 16 hydrogen bonds · protein-protein
• A–B · 1852 Ų buried · 15 hydrogen bonds · protein-protein
• L–A · 518 Ų buried · 9 hydrogen bonds · protein-protein
• H–B · 302 Ų buried · 3 hydrogen bonds · protein-protein
• H–A · 181 Ų buried · 2 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 10 disulfide (disulfide).

Compared structures

• 2G2R · Green-fluorescent antibody 11G10 in complex with its hapten (nitro-stilbene derivative) · the same entry in another collection · chain L against L, TM-score 1.00
• P84751 · AlphaFold monomer V2.0 prediction for ig heavy chain MEM5 (P84751) · a predicted model of the same protein · chain H against A, TM-score 0.92

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P84751
• Elsewhere: RCSB PDB 2G2R · PDBe 2G2R · UniProt P84751