MolNFT v1 · #26519 · protein
Metal ion dependency of the antiterminator protein, hutp, for binding to the terminator region of hut mRNA- a structural basis
1WRN · Hut operon positive regulatory protein: “Metal ion dependency of the antiterminator protein, hutp, for binding to the terminator region of hut mRNA- a…”. X-ray diffraction, 2 ligands (HIS, MN), 5 pockets (best druggability 0.69).…
- PDB
- 1WRN
- Type
- protein
- Length
- 435 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Hut operon positive regulatory protein
- UniProt
- P10943
- Ligands
- HIS, MN
- Pockets
- 5 · best druggability 0.69
Structure
x-ray diffraction · 2 distinct chains · 38% helix, 30% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, B | Hut operon positive regulatory protein | protein | 146 | 35% helix, 30% strand | P10943 |
| C | Hut operon positive regulatory protein | protein | 143 | 41% helix, 31% strand | P10943 |
Ligands and binding sites
- HIS · Histidine · ligand · 3 copies · chains A, C, B
- MN · Manganese (II) ion · metal · 3 copies · chains A, B, C
- PEG · Di(hydroxyethyl)ether · additive · 6 copies · chains A, C, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
5 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 65 | 0.69 | 0.96 | 20 | HIS |
| 2 | 91 | 0.68 | 0.92 | 24 | PEG, HIS |
| 3 | 132 | 0.64 | 0.87 | 28 | MN, HIS |
| 4 | 169 | 0.50 | 0.78 | 28 | empty |
| 5 | 57 | 0.36 | 0.79 | 12 | empty |
Interfaces
3 interfaces between chains.
- A–B · 564 Ų buried · 4 hydrogen bonds · protein-protein
- A–C · 555 Ų buried · 4 hydrogen bonds · protein-protein
- B–C · 545 Ų buried · 3 hydrogen bonds · protein-protein
Compared structures
- 1WRN · Metal Ion dependency of the antiterminator protein, HutP, for binding to the terminator… · the same entry in another collection · chain B against B, TM-score 1.00
- P10943 · AlphaFold monomer V2.0 prediction for hut operon positive regulatory protein (P10943) · a predicted model of the same protein · chain C against A, TM-score 0.99
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P10943
- Elsewhere: RCSB PDB 1WRN · PDBe 1WRN · UniProt P10943
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.