MolNFT v1 · #22538 · protein
Crystal structure of the ferrous co-bound cytochrome P450CAM (C334A)
1T87 · Cytochrome P450-cam: “Crystal structure of the ferrous co-bound cytochrome P450CAM (C334A)”. X-ray diffraction, 4 ligands (HEM, CAM, K), 5 pockets (best druggability 0.87). MolNFT v1 #22538 on GenesisL1: 3D structure, binding sites…
- PDB
- 1T87
- Type
- protein
- Length
- 812 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Cytochrome P450-cam
- EC
- 1.14.15.1
- UniProt
- P00183
- Ligands
- HEM, CAM, K, CMO
- Pockets
- 5 · best druggability 0.87
Structure
x-ray diffraction · 2 distinct chains · 52% helix, 11% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Cytochrome P450-cam (EC 1.14.15.1) | protein | 407 | 52% helix, 10% strand | P00183 |
| B | Cytochrome P450-cam (EC 1.14.15.1) | protein | 405 | 52% helix, 11% strand | P00183 |
Ligands and binding sites
- HEM · Protoporphyrin IX containing Fe · ligand · 2 copies · chains A, B
- CAM · Camphor · ligand · 2 copies · chains A, B
- K · Potassium ion · metal · 3 copies · chains A, B
- CMO · Carbon monoxide · ion · chains B
- TRS · 2-amino-2-hydroxymethyl-propane-1,3-diol · additive · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
5 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 449 | 0.87 | 0.93 | 48 | HEM, CMO, CAM |
| 2 | 440 | 0.86 | 0.93 | 51 | HEM, CAM |
| 3 | 719 | 0.71 | 0.78 | 61 | K, TRS |
| 4 | 101 | 0.37 | 0.75 | 20 | empty |
| 5 | 53 | 0.32 | 0.77 | 15 | empty |
Interfaces
1 interface between chains.
- A–B · 322 Ų buried · 3 hydrogen bonds · protein-protein
Compared structures
- 1T87 · Crystal Structure of the Ferrous CO-bound Cytochrome P450cam (C334A) · the same entry in another collection · chain A against A, TM-score 1.00
- P00183 · AlphaFold monomer V2.0 prediction for camphor 5-monooxygenase (P00183) · a predicted model of the same protein · chain B against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P00183
- Elsewhere: RCSB PDB 1T87 · PDBe 1T87 · UniProt P00183
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.