MolNFT v1 · #189034 · protein + RNA
Staphylococcus epidermidis RP62A CRISPR effector complex with non-self target RNA 2
7UZY · CRISPR system single-strand-specific deoxyribonuclease…: “Staphylococcus epidermidis RP62A CRISPR effector complex with non-self target RNA 2”. Electron microscopy, 20 pockets (best druggability 0.83). MolNFT v1 #189034 on…
- PDB
- 7UZY
- Type
- protein + RNA
- Length
- 1,825 residues
- Chains
- 11
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR system single-strand-specific deoxyribonuclease CAS10/CSM1 (subtype III-a); CRISPR…
- UniProt
- Q5HK89, Q5HK90, Q5HK91, Q5HK92
- Pockets
- 20 · best druggability 0.83
Structure
electron microscopy · 10 distinct chains · 42% helix, 20% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| F | CRISPR system single-strand-specific deoxyribonuclease CAS10/CSM1 (subtype III-a) | protein | 414 | 55% helix, 12% strand | Q5HK89 |
| J | CRISPR system cms protein CSM2 | protein | 102 | 77% helix, 4% strand | Q5HK90 |
| I | CRISPR system cms protein CSM2 | protein | 110 | 80% helix, 2% strand | Q5HK90 |
| K | CRISPR system cms protein CSM2 | protein | 59 | 85% helix, 0% strand | Q5HK90 |
| D | CRISPR system cms endoribonuclease CSM3 | protein | 198 | 27% helix, 32% strand | Q5HK91 |
| C, A | CRISPR system cms endoribonuclease CSM3 | protein | 205 | 29% helix, 30% strand | Q5HK91 |
| B | CRISPR system cms endoribonuclease CSM3 | protein | 192 | 25% helix, 32% strand | Q5HK91 |
| H | CRISPR system cms protein CSM4 | protein | 296 | 22% helix, 22% strand | Q5HK92 |
| G | RNA (37-mer) | rna | 30 | — | — |
| L | RNA (40-mer) | rna | 14 | — | — |
Pockets
20 pockets found on a 1 Å grid, 16 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1188 | 0.83 | 0.84 | 104 | empty |
| 2 | 965 | 0.78 | 0.80 | 89 | empty |
| 3 | 919 | 0.75 | 0.79 | 70 | empty |
| 4 | 612 | 0.75 | 0.82 | 63 | empty |
| 5 | 355 | 0.72 | 0.84 | 36 | empty |
Interfaces
26 interfaces between chains.
- H–G · 1552 Ų buried · 25 hydrogen bonds · protein-nucleic
- A–B · 1385 Ų buried · 13 hydrogen bonds · protein-protein
- C–A · 1324 Ų buried · 16 hydrogen bonds · protein-protein
- D–C · 1281 Ų buried · 7 hydrogen bonds · protein-protein
- B–H · 1227 Ų buried · 11 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 2 disulfide (disulfide).
Compared structures
- 7UZY · Staphylococcus epidermidis RP62A CRISPR effector complex with non-self target RNA 2 · the same entry in another collection · chain F against F, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q5HK89
- Every structure of UniProt Q5HK90
- Every structure of UniProt Q5HK91
- Every structure of UniProt Q5HK92
- Elsewhere: RCSB PDB 7UZY · PDBe 7UZY · UniProt Q5HK89 · UniProt Q5HK90 · UniProt Q5HK91 · UniProt Q5HK92
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