MolNFT v1 · #189033 · protein + RNA
Staphylococcus epidermidis RP62A CRISPR effector subcomplex with non- self target RNA bound
7UZX · CRISPR system cms endoribonuclease CSM3: “Staphylococcus epidermidis RP62A CRISPR effector subcomplex with non- self target RNA bound”. Electron microscopy, 20 pockets (best druggability 0.81). MolNFT v1 #189033 on GenesisL1: 3D…
- PDB
- 7UZX
- Type
- protein + RNA
- Length
- 1,796 residues
- Chains
- 9
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR system cms endoribonuclease CSM3; CRISPR system cms protein CSM4; CRISPR system…
- UniProt
- Q5HK91, Q5HK92, Q5HK89
- Pockets
- 20 · best druggability 0.81
Structure
electron microscopy · 7 distinct chains · 36% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, C, D | CRISPR system cms endoribonuclease CSM3 | protein | 204 | 26% helix, 33% strand | Q5HK91 |
| B | CRISPR system cms endoribonuclease CSM3 | protein | 193 | 26% helix, 32% strand | Q5HK91 |
| E | CRISPR system cms endoribonuclease CSM3 | protein | 184 | 29% helix, 24% strand | Q5HK91 |
| H | CRISPR system cms protein CSM4 | protein | 293 | 24% helix, 23% strand | Q5HK92 |
| F | CRISPR system single-strand-specific deoxyribonuclease CAS10/CSM1 (subtype III-a) | protein | 478 | 53% helix, 10% strand | Q5HK89 |
| G | Staphylococcus epidermidis RP62A CRISPR RNA: repeat plus spacer sequence 2 | rna | 31 | — | — |
| L | CRISPR non-self RNA target | rna | 5 | — | — |
Pockets
20 pockets found on a 1 Å grid, 18 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 803 | 0.81 | 0.84 | 63 | empty |
| 2 | 737 | 0.77 | 0.82 | 92 | empty |
| 3 | 66 | 0.74 | 0.97 | 19 | empty |
| 4 | 384 | 0.72 | 0.83 | 37 | empty |
| 5 | 408 | 0.70 | 0.82 | 48 | empty |
Interfaces
21 interfaces between chains.
- H–G · 1566 Ų buried · 23 hydrogen bonds · protein-nucleic
- D–E · 1464 Ų buried · 16 hydrogen bonds · protein-protein
- C–D · 1374 Ų buried · 10 hydrogen bonds · protein-protein
- A–C · 1367 Ų buried · 13 hydrogen bonds · protein-protein
- A–B · 1332 Ų buried · 11 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 1 disulfide (disulfide).
Compared structures
- 7UZX · Staphylococcus epidermidis RP62a CRISPR effector subcomplex with non-self target RNA bound · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q5HK91
- Every structure of UniProt Q5HK92
- Every structure of UniProt Q5HK89
- Elsewhere: RCSB PDB 7UZX · PDBe 7UZX · UniProt Q5HK91 · UniProt Q5HK92 · UniProt Q5HK89
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