E.COLI'S putrescine receptor variant POTF/D (4JDF) with mutations E39D F88L in complex with spermidine

Structure

x-ray diffraction · 1 distinct chain · 49% helix, 18% strand (DSSP).

Ligands and binding sites

• SPD · Spermidine · ligand · chains A
• CL · Chloride ion · ion · chains A
• EDO · 1,2-ethanediol · additive · 17 copies · chains A
• GOL · Glycerol · additive · 2 copies · chains A
• PEG · Di(hydroxyethyl)ether · additive · 2 copies · chains A
• ACT · Acetate ion · additive · chains A
• PGE · Triethylene glycol · additive · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

2 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Modifications

Observed in the structure: 2 covalent, 1 disulfide (additive adduct, disulfide).

Compared structures

• 7OYT · E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88L in complex… · the same entry in another collection · chain A against A, TM-score 1.00
• P31133 · AlphaFold monomer V2.0 prediction for putrescine-binding periplasmic protein potf (P31133) · a predicted model of the same protein · chain A against A, TM-score 0.97

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P31133
• Elsewhere: RCSB PDB 7OYT · PDBe 7OYT · UniProt P31133